由四氨合钴 -ATP诱导产生新亚基相互作用的钠钾 -ATP酶二维晶体阵列
Two-dimensional crystalline arrays of Na,K-ATPase with new subunit interactions induced by cobalt-tetrammine-ATP.
作者信息
Skriver E, Maunsbach A B, Hebert H, Scheiner-Bobis G, Schoner W
机构信息
Department of Cell Biology, University of Aarhus, Denmark.
出版信息
J Ultrastruct Mol Struct Res. 1989 Dec;102(3):189-95. doi: 10.1016/0889-1605(89)90013-x.
PMID:2561364
Abstract
Purified membrane-bound Na,K-ATPase incubated with cobalt-tetrammine-ATP [Co(NH3)4ATP], which is a stable MgATP complex analog, shows two new types of membrane crystals, a new p21 form and a p4 form. The building blocks of the crystalline arrays correspond to (alpha beta)2 dimers of the enzyme protein suggesting that alpha-alpha interaction may be important in the pumping process.
摘要
用钴四氨ATP[Co(NH₃)₄ATP]（一种稳定的MgATP复合物类似物）孵育纯化的膜结合钠钾ATP酶，会出现两种新型膜晶体，一种新的p21形式和一种p4形式。晶体阵列的结构单元对应于酶蛋白的(αβ)₂二聚体，这表明α-α相互作用在泵浦过程中可能很重要。
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