酵母提取物成分吡咯喹啉醌和天冬氨酸对肺炎克雷伯菌IFO 13541中维生素B12产生的影响。

Effects of the yeast extract components pyrroloquinoline quinone and aspartic acid on vitamin B12 production in Klebsiella pneumoniae IFO 13541.

作者信息

Ohsugi M, Noda H, Muro K, Ishiba A, Kondo Y, Nakao S

机构信息

Department of Food Science, Faculty of Home Economics, Mukogawa Women's University, Nishinomiya, Japan.

出版信息

J Nutr Sci Vitaminol (Tokyo). 1989 Dec;35(6):661-5. doi: 10.3177/jnsv.35.661.

DOI:10.3177/jnsv.35.661

PMID:2561367

Abstract

The production of vitamin B12 from carbohydrates, peptone, casamino acid, etc., by intestinal bacteria was investigated. Klebsiella pneumoniae IFO 13541 was the most efficient strain for vitamin B12 production, which depended exclusively on the concentration of yeast extract added to the medium. A concentrated solution of yeast extract (1 ml) was chromatographed on a Sephadex G-25 column (1 x 180 cm) and eluted with H2O (eighty fractions of 3 ml each were collected). It was found that fractions in which bacterial growth was most prevalent also exhibited the highest amount of vitamin production. The effectiveness of yeast extract was shown by the participation of pyrroloquinoline quinone and aspartic acid in the growth stimulation and in the vitamin B12 production in this strain.

摘要

研究了肠道细菌利用碳水化合物、蛋白胨、酪蛋白氨基酸等生产维生素B12的情况。肺炎克雷伯菌IFO 13541是生产维生素B12最有效的菌株，其产量完全取决于添加到培养基中的酵母提取物浓度。将酵母提取物浓缩液（1毫升）在Sephadex G - 25柱（1×180厘米）上进行色谱分析，并用蒸馏水洗脱（共收集80个3毫升的馏分）。结果发现，细菌生长最旺盛的馏分中维生素产量也最高。吡咯喹啉醌和天冬氨酸参与该菌株的生长刺激及维生素B12的生产，这表明了酵母提取物的有效性。