Zhu Bei, Wang Cuiyu, Zhang Xiaoli, He Juan, Wu Jianqing, Yu Rongbin, Zhao Weihong
Department of Geriatrics, The First Affiliated Hospital of Nanjing Medical University , Nanjing, Jiangsu , China and.
Ren Fail. 2015 Apr;37(3):505-10. doi: 10.3109/0886022X.2015.1006086. Epub 2015 Jan 23.
Hepatitis C virus (HCV) commonly causes a chronic infection but portion of hemodialysis patients are able to resistant to HCV infection, even clear the virus naturally. Interleukin-10 (IL-10) and interleukin-12 (IL-12) are crucial regulators of the immune response to T-helper 1 (Th1) or T-helper 2 (Th2) categories and play a role in autoimmune and infectious diseases. This study was undertaken to investigate the possible association of genetic polymorphisms of 3' untranslated region (3'UTR) of the IL-12B (-1188) and IL-10 (-592 and -819) and hepatitis C in Chinese Han hemodialysis (HD) patients.
The genotyping of IL-12B 3'UTR and IL-10 -592 and -819 were performed by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method.
Compared with the IL-12B-AA genotype, CC and combined CC/AC genotypes were associated with a significant decreased risk of HCV infection in Chinese hemodialysis patients (p < 0.001). However, the IL-10-592 and -819 genotypes were not found significant difference both between the anti-HCV (+) and anti-HCV (-) patients (p > 0.05) and the persistent infection and viral clearance HD patients (p > 0.05).
The present study indicated that the polymorphisms of IL-12B 3'UTR might contribute to the susceptibility of HCV infection in Chinese HD population.
丙型肝炎病毒（HCV）通常引起慢性感染，但部分血液透析患者能够抵抗HCV感染，甚至自然清除病毒。白细胞介素-10（IL-10）和白细胞介素-12（IL-12）是免疫反应对辅助性T细胞1（Th1）或辅助性T细胞2（Th2）类别至关重要的调节因子，在自身免疫性疾病和感染性疾病中发挥作用。本研究旨在探讨白细胞介素-12B（IL-12B）基因3'非翻译区（3'UTR）（-1188）和白细胞介素-10（IL-10）基因（-592和-819）的基因多态性与中国汉族血液透析（HD）患者丙型肝炎的可能关联。
采用聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）方法对IL-12B基因3'UTR及IL-10基因-592和-819进行基因分型。
与IL-12B-AA基因型相比，CC基因型及CC/AC联合基因型与中国血液透析患者HCV感染风险显著降低相关（p < 0.001）。然而，IL-10基因-592和-819基因型在抗-HCV（+）和抗-HCV（-）患者之间（p > 0.05）以及持续性感染和病毒清除的HD患者之间（p > 0.05）均未发现显著差异。
本研究表明，IL-12B基因3'UTR的多态性可能与中国HD人群HCV感染的易感性有关。
