Mäntynen Sari, Laanto Elina, Kohvakka Annika, Poranen Minna M, Bamford Jaana K H, Ravantti Janne J
Department of Biological and Environmental Science and Nanoscience Center, University of Jyväskylä, Jyväskylä, Finland.
Department of Biosciences, University of Helsinki, Helsinki, Finland.
J Gen Virol. 2015 May;96(Pt 5):1180-1189. doi: 10.1099/vir.0.000063. Epub 2015 Jan 22.
Cystoviridae is a family of bacteriophages with a tri-segmented dsRNA genome enclosed in a tri-layered virion structure. Here, we present a new putative member of the Cystoviridae family, bacteriophage ϕNN. ϕNN was isolated from a Finnish lake in contrast to the previously identified cystoviruses, which originate from various legume samples collected in the USA. The nucleotide sequence of the virus reveals a strong genetic similarity (~80 % for the L-segments, ~55 % for the M-segments and ~84 % for the S-segments) to Pseudomonas phage ϕ6, the type member of the virus family. However, the relationship between ϕNN and other cystoviruses is more distant. In general, proteins located in the internal parts of the virion were more conserved than those exposed on the virion surface, a phenomenon previously reported among eukaryotic dsRNA viruses. Structural models of several putative ϕNN proteins propose that cystoviral structures are highly conserved.
囊病毒科是一类噬菌体，其基因组为三段双链RNA，包裹在三层病毒粒子结构中。在此，我们展示了囊病毒科的一个新的假定成员——噬菌体ϕNN。与之前鉴定的囊病毒不同，ϕNN是从芬兰的一个湖泊中分离出来的，之前鉴定的囊病毒源自在美国采集的各种豆科植物样本。该病毒的核苷酸序列显示，它与病毒科的典型成员假单胞菌噬菌体ϕ6有很强的遗传相似性（L片段约80%，M片段约55%，S片段约84%）。然而，ϕNN与其他囊病毒的关系更为疏远。一般来说，位于病毒粒子内部的蛋白质比暴露在病毒粒子表面的蛋白质更保守，这种现象之前在真核双链RNA病毒中也有报道。几种假定的ϕNN蛋白质的结构模型表明，囊病毒结构高度保守。
