Nnalue N A, Stocker B A
Department of Microbiology and Immunology, Stanford University, California 94305.
J Gen Microbiol. 1989 Dec;135(12):3345-52. doi: 10.1099/00221287-135-12-3345.
We tested the transfer of several plasmids and transposons from Escherichia coli to Pasteurella multocida by filter mating. Two plasmids, pRKTV5 (pRK2013::Tn7) and pUW964 (pRKTV5::Tn5), were derived from pRK2013--a narrow-host-range plasmid with the broad-host-range IncP conjugation genes. Most P. multocida transconjugants obtained with pRKTV5 had Tn7 insertions in the chromosome but some had insertions of the whole plasmid. By contrast, all the transconjugants obtained with pUW964 had insertions of this plasmid or a deleted variant. pUW964 mediated low-frequency transfer of Tn7 or chromosomal markers between P. multocida strains. Broad-host-range IncP plasmid RP4 (RK2) did not yield selectable transconjugants in P. multocida but two plasmids derived by Tn5 insertion into a kanamycin-sensitive derivative of RP4 did yield transconjugants. pSUP1011, a narrow-host-range p15A replicon with the RP4 mob region allowing mobilization by the IncP conjugation genes also yielded transconjugants while several other plasmids tested did not transfer markers to P. multocida.
我们通过滤膜杂交试验检测了几种质粒和转座子从大肠杆菌向多杀巴斯德菌的转移情况。两种质粒,pRKTV5(pRK2013::Tn7)和pUW964(pRKTV5::Tn5),源自pRK2013——一种带有广宿主范围IncP接合基因的窄宿主范围质粒。用pRKTV5获得的大多数多杀巴斯德菌转接合子在染色体中有Tn7插入,但有些有整个质粒的插入。相比之下,用pUW964获得的所有转接合子都有该质粒或缺失变体的插入。pUW964介导了Tn7或染色体标记在多杀巴斯德菌菌株之间的低频转移。广宿主范围的IncP质粒RP4(RK2)在多杀巴斯德菌中未产生可选择的转接合子,但通过Tn5插入到RP4的卡那霉素敏感衍生物中得到的两种质粒确实产生了转接合子。pSUP1011,一种带有RP4 mob区域的窄宿主范围p15A复制子,允许通过IncP接合基因进行转移,也产生了转接合子,而测试的其他几种质粒未将标记转移到多杀巴斯德菌中。