一种可通过电穿孔和接合作用在大肠杆菌和溶血巴斯德氏菌之间转移的质粒。

A plasmid which can be transferred between Escherichia coli and Pasteurella haemolytica by electroporation and conjugation.

作者信息

Craig F F, Coote J G, Parton R, Freer J H, Gilmour N J

机构信息

Microbiology Department, University of Glasgow, UK.

出版信息

J Gen Microbiol. 1989 Nov;135(11):2885-90. doi: 10.1099/00221287-135-11-2885.

DOI:10.1099/00221287-135-11-2885

PMID:2693589

Abstract

Three broad-host-range plasmids (pRK290, pSa4 and pKT230) and one native Pasteurella haemolytica plasmid (pPH33) were used in transformation experiments with P. haemolytica strains T179 (serotype A1), Y216 (serotype A2) and its capsular-deficient variant Y216/NS1. No transformants were detected with either heat-shock or freeze-thaw techniques. However, by electroporation, all P. haemolytica strains were transformed by pPH33 but not by pRK290 or pSa4. The highest frequency obtained was 91 x 10(4) transformants per microgram of pPH33 DNA with P. haemolytica strain Y216/NS1. Although pPH33 itself was non-transmissible by conjugation, it could be mobilized from Escherichia coli, using the transfer function of the IncP plasmid pRK2013, into P. haemolytica at a frequency of 0.3-2.2 x 10(-3) per recipient cell.

摘要

三种广宿主范围质粒（pRK290、pSa4和pKT230）以及一种溶血巴斯德氏菌天然质粒（pPH33）用于对溶血巴斯德氏菌菌株T179（血清型A1）、Y216（血清型A2）及其荚膜缺陷型变体Y216/NS1进行转化实验。采用热休克或冻融技术均未检测到转化子。然而，通过电穿孔法，所有溶血巴斯德氏菌菌株均被pPH33转化，但未被pRK290或pSa4转化。获得的最高转化频率是每微克pPH33 DNA产生91×10⁴个转化子，所用的溶血巴斯德氏菌菌株为Y216/NS1。尽管pPH33本身不能通过接合进行转移，但利用IncP质粒pRK2013的转移功能，它可以从大肠杆菌转移至溶血巴斯德氏菌，转移频率为每个受体细胞0.3 - 2.2×10⁻³。