Zhou Hao, Chen Shun, Qi Yulin, Zhou Qin, Wang Mingshu, Jia Renyong, Zhu Dekang, Liu Mafeng, Sun Kunfeng, Liu Fei, Chen Xiaoyue, Cheng Anchun
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China; Avian Disease Research Center, College of Veterinary Medicine of Sichuan Agricultural University, Ya'an, Sichuan 625014, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, PR China.
Gene. 2015 Apr 25;561(1):35-44. doi: 10.1016/j.gene.2015.01.040. Epub 2015 Jan 21.
The cDNAs encoding two distinct type I interferon receptors were firstly cloned from the spleen of white goose (the Chinese goose, Anser cygnoides). The cDNA of goose IFNAR1 consisted of 1616 bp and encoded 406 amino acids with a predicted molecular weight of 46.4 kDa, while the cDNA of goose IFNAR2 consisted of 1525 bp and encoded 294 amino acids with a predicted molecular weight of 32.6 kDa. The IFNAR1 shared 85.4% identity in deduced amino acid sequence with duck IFNAR1, while IFNAR2 amino acid sequence showed 86% identity with that of duck IFNAR2. The age-related analysis of gene expression revealed that goose IFNα and IFNARs were all highly transcribed in pancreas, which may due to a reasonable amount of dendritic cells aggregated in pancreas. And goose IFNα and its cognate receptors had different structural features and tissue expression patterns during the period from embryonic goose to adult goose, suggesting that IFNα and IFNARs may maintain a developmental dynamic immune competence in unstimulated states. The data provided in this study may contribute to future understanding of the interaction between interferon and interferon receptors in immune mechanism. And it also helps us to understand the age-related susceptibility to pathogens in birds better.
编码两种不同的I型干扰素受体的cDNA首次从白鹅（中国鹅，鸿雁）的脾脏中克隆出来。鹅IFNAR1的cDNA由1616个碱基对组成，编码406个氨基酸，预测分子量为46.4 kDa，而鹅IFNAR2的cDNA由1525个碱基对组成，编码294个氨基酸，预测分子量为32.6 kDa。IFNAR1在推导的氨基酸序列中与鸭IFNAR1有85.4%的同一性，而IFNAR2的氨基酸序列与鸭IFNAR2的氨基酸序列有86%的同一性。基因表达的年龄相关性分析表明，鹅IFNα和IFNARs在胰腺中均高度转录，这可能是由于胰腺中聚集了适量的树突状细胞。并且从胚胎期鹅到成年鹅期间，鹅IFNα及其同源受体具有不同的结构特征和组织表达模式，表明IFNα和IFNARs可能在未受刺激状态下维持发育动态免疫能力。本研究提供的数据可能有助于未来理解干扰素与干扰素受体在免疫机制中的相互作用。它也有助于我们更好地理解鸟类对病原体的年龄相关性易感性。
