高蛋白饮食诱导大鼠脑内氧化应激：高强度运动对脂质过氧化的保护作用。

High-protein diet induces oxidative stress in rat brain: protective action of high-intensity exercise against lipid peroxidation.

作者信息

Camiletti-Moirón Daniel, Aparicio Virginia Arianna, Nebot Elena, Medina Gerardo, Martínez Rosario, Kapravelou Garyfallia, Andrade Ana, Porres Jesús María, López-Jurado María, Aranda Pilar

机构信息

Department of Physiology. Faculty of Pharmacy, Faculty of Sport Sciences, and Institute of Nutrition and Food Technology, The Joint Institute for Sport and Health. University of Granada. Spain..

出版信息

Nutr Hosp. 2014 Oct 25;31(2):866-74. doi: 10.3305/nh.2015.31.2.8182.

DOI:10.3305/nh.2015.31.2.8182

PMID:25617575

Abstract

INTRODUCTION

It is well established that soy protein diets as well as aerobic exercise could promote antioxidant capacity and consequently reduce free radicals overproduction on brain. However, little is know regarding to the high-protein diets and high intensity exercise on oxidative stress production. The aim of this study was to analyse the effects of high-protein diets and high-intensity exercise (HIE) on brain oxidative stress markers.

MATERIALS AND METHODS

A total of 40 male Wistar rats were randomly distributed in 4 experimental groups (n=10): normal-protein or high-protein diets with or without HIE for an experimental period of 12 weeks. Main oxidative damage markers in brain such as thiobarbituric acid-reactive substances (TBARs) and protein carbonyl content (PCC) were assessed. In addition, brain manganese superoxide dismutase (Mn-SOD), cooper/ zinc superoxide dismutase (CuZn-SOD) and catalase (CAT) antioxidant enzymes activity, and protein level of Nuclear factor erythroid 2 related factor 2 (Nrf2) were measured.

RESULTS AND DISCUSSION

Brain TBARs, PCC, tSOD, Mn-SOD, CuZn-SOD and CAT levels were higher in the high-protein compared to the normal-protein groups (all, p.

摘要

引言

众所周知，大豆蛋白饮食以及有氧运动能够提高抗氧化能力，从而减少大脑中自由基的过量产生。然而，关于高蛋白饮食和高强度运动对氧化应激产生的影响却知之甚少。本研究的目的是分析高蛋白饮食和高强度运动（HIE）对大脑氧化应激标志物的影响。

材料与方法

总共40只雄性Wistar大鼠被随机分为4个实验组（n = 10）：正常蛋白或高蛋白饮食，有或没有进行高强度运动，实验期为12周。评估大脑中的主要氧化损伤标志物，如硫代巴比妥酸反应性物质（TBARs）和蛋白质羰基含量（PCC）。此外，还测量了大脑锰超氧化物歧化酶（Mn-SOD）、铜/锌超氧化物歧化酶（CuZn-SOD）和过氧化氢酶（CAT）的抗氧化酶活性，以及核因子红细胞2相关因子2（Nrf2）的蛋白质水平。