Rojas J J, Deniz B F, Schuch C P, Carletti J V, Deckmann I, Diaz R, Matté C, dos Santos T M, Wyse A T, Netto C A, Pereira L O
Programa de Pós-graduação em Neurociências, ICBS, Universidade Federal do Rio Grande do Sul, Brazil; Departamento de Ciências Morfológicas, ICBS, Universidade Federal do Rio Grande do Sul, Brazil.
Departamento de Ciências Morfológicas, ICBS, Universidade Federal do Rio Grande do Sul, Brazil.
Neuroscience. 2015 Apr 16;291:118-27. doi: 10.1016/j.neuroscience.2015.01.017. Epub 2015 Jan 21.
In animal models, environmental enrichment (EE) has been found to be an efficient treatment for alleviating the consequences of neonatal hypoxia-ischemia (HI). However the potential for this therapeutic strategy and the mechanisms involved are not yet clear. The aim of present study is to investigate behavioral performance in the ox-maze test and Na+,K+-ATPase, catalase (CAT) and glutathione peroxidase (GPx) activities in the hippocampus of rats that suffered neonatal HI and were stimulated in an enriched environment. Seven-day-old rats were submitted to the HI procedure and divided into four groups: control maintained in standard environment (CTSE), control submitted to EE (CTEE), HI in standard environment (HISE) and HI in EE (HIEE). Animals were stimulated with EE for 9 weeks (1 h/day for 6 days/week) and then behavioral and biochemical parameters were evaluated. Present results indicate learning and memory in the ox-maze task were impaired in HI rats and this effect was recovered after EE. Hypoxic-ischemic event did not alter the Na+,K+-ATPase activity in the right hippocampus (ipsilateral to arterial occlusion). However, on the contralateral hemisphere, HI caused a decrease in this enzyme activity that was recovered by EE. The activities of GPx and CAT were not changed by HI in any group evaluated. In conclusion, EE was effective in recovering learning and memory impairment in the ox-maze task and Na+,K+-ATPase activity in the hippocampus caused by HI. The present data provide further support for the therapeutic potential of environmental stimulation after neonatal HI in rats.
在动物模型中，发现环境富集（EE）是减轻新生儿缺氧缺血（HI）后果的一种有效治疗方法。然而，这种治疗策略的潜力及其涉及的机制尚不清楚。本研究的目的是调查新生期HI且在丰富环境中受到刺激的大鼠在牛津迷宫试验中的行为表现以及海马中钠钾ATP酶、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性。将7日龄大鼠进行HI手术，并分为四组：饲养在标准环境中的对照组（CTSE）、接受EE的对照组（CTEE）、饲养在标准环境中的HI组（HISE）和饲养在EE中的HI组（HIEE）。用EE刺激动物9周（每天1小时，每周6天），然后评估行为和生化参数。目前的结果表明，HI大鼠在牛津迷宫任务中的学习和记忆受损，而EE后这种影响得到恢复。缺氧缺血事件并未改变右侧海马（与动脉闭塞同侧）中的钠钾ATP酶活性。然而，在对侧半球，HI导致该酶活性降低，而EE使其恢复。在任何评估组中，HI均未改变GPx和CAT的活性。总之，EE可有效恢复HI所致的牛津迷宫任务中的学习和记忆障碍以及海马中的钠钾ATP酶活性。本数据为新生期HI后大鼠环境刺激的治疗潜力提供了进一步支持。
