Ge Yong-Sheng, Liu Dong, Jia Wei-Dong, Li Jian-Sheng, Ma Jin-Liang, Yu Ji-Hai, Xu Ge-Liang
Graduate School of Tianjin Medical University, Tianjin 300070, China; Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, Anhui Provincial Hospital, Hefei 230001, Anhui Province, China; Department of Hepatic Surgery, Anhui Provincial Hospital, Anhui Medical University, Hefei 230001, Anhui Province, China.
Anhui Province Key Laboratory of Hepatopancreatobiliary Surgery, Anhui Provincial Hospital, Hefei 230001, Anhui Province, China; Department of general surgery, Lu'an People's Hospital, Lu'an 237005, Anhui Province, China.
Pathol Res Pract. 2015 Mar;211(3):198-202. doi: 10.1016/j.prp.2014.09.011. Epub 2014 Oct 2.
The association of aberrant expression of Kindlin-2 with tumor progression has been reported in recent years. The purpose of this study was to investigate the expression of Kindlin-2 in hepatocellular carcinoma (HCC), and to evaluate its clinical and prognostic significance. The mRNA and protein levels of Kindlin-2 in HCC and adjacent non-cancerous tissues were examined by real-time PCR and western blotting. The relationships between Kindlin-2 expression, clinicopathological features and postoperative survival of HCC patients were also evaluated. Kindlin-2 expression was higher in HCC tissues as compared to adjacent non-cancerous tissues at both mRNA and protein levels (P<0.05, respectively). Positive expression of Kindlin-2 was significantly correlated with larger tumor size (P=0.034), capsular invasion (P=0.009), microvascular invasion (P=0.028) and poor prognosis of HCC patients (P<0.001). Moreover, multivariate survival analysis identified Kindlin-2 as an independent prognostic factor for overall and disease-free survival of HCC patients (P=0.018 and 0.001, respectively). Taken together, our findings suggested that Kindlin-2 was highly expressed in HCC tissues and was closely related to clinical progression. Therefore, Kindlin-2 protein could be a potential biomarker for predicting poor prognosis of HCC patients after surgery.
近年来，已有报道称Kindlin-2的异常表达与肿瘤进展相关。本研究的目的是调查Kindlin-2在肝细胞癌（HCC）中的表达情况，并评估其临床及预后意义。通过实时定量PCR和蛋白质印迹法检测HCC组织及癌旁非癌组织中Kindlin-2的mRNA和蛋白质水平。同时评估Kindlin-2表达与HCC患者临床病理特征及术后生存情况之间的关系。在mRNA和蛋白质水平上，HCC组织中Kindlin-2的表达均高于癌旁非癌组织（P均<0.05）。Kindlin-2的阳性表达与肿瘤较大尺寸（P=0.034）、包膜侵犯（P=0.009）、微血管侵犯（P=0.028）及HCC患者预后不良（P<0.001）显著相关。此外，多因素生存分析确定Kindlin-2是HCC患者总生存和无病生存的独立预后因素（P分别为0.018和0.001）。综上所述，我们的研究结果表明Kindlin-2在HCC组织中高表达，且与临床进展密切相关。因此，Kindlin-2蛋白可能是预测HCC患者术后预后不良情况的潜在生物标志物。
