Fátima Barroso M, Freitas Maria, Oliveira M Beatriz P P, de-Los-Santos-Álvarez Noemí, Lobo-Castañón María Jesús, Delerue-Matos Cristina
REQUIMTE, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal.
REQUIMTE, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida 431, 4200-072 Porto, Portugal; Dpto. Química Física y Analítica, Universidad de Oviedo, Av. Julián Clavería 8, 33006 Oviedo, Spain.
Talanta. 2015 Mar;134:158-164. doi: 10.1016/j.talanta.2014.10.017. Epub 2014 Oct 18.
In the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis.
在本研究中，提出了一种用于事件特异性检测MON810转基因玉米的基因传感器。利用纳米结构化技术，制备了一种具有成本效益的三维电极，并对包含二硫醇、单硫醇和硫醇化捕获探针的三元单层进行了优化，以最小化非特异性信号。选择夹心式检测方法以排除与稳定二级靶标结构相关的低效杂交。金纳米结构化电极与市售丝网印刷电极的分析性能比较突出了纳米结构化电极的优越性能。最后，该基因传感器有效地应用于实际样品中转基因序列的检测，显示了其在未来定量分析中的潜力。
