Erden Pınar Esra, Kaçar Ceren, Öztürk Funda, Kılıç Esma
Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey.
Department of Chemistry, Faculty of Science, Ankara University, Ankara, Turkey.
Talanta. 2015 Mar;134:488-495. doi: 10.1016/j.talanta.2014.11.058. Epub 2014 Dec 2.
In this study, a new uric acid biosensor was constructed based on ferrocene containing polymer poly(vinylferrocene) (PVF), carboxylated multiwalled carbon nanotubes (c-MWCNT) and gelatin (GEL) modified glassy carbon electrode (GCE). Uricase enzyme (UOx) was immobilized covalently through N-ethyl-N'-(3-dimethyaminopropyl) carbodiimide (EDC) and N-hydroxyl succinimide (NHS) chemistry onto c-MWCNT/GEL/PVF/GCE. The c-MWCNT/GEL/PVF composite was characterized by scanning electron microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. Various experimental parameters such as pH, applied potential, enzyme loading, PVF and c-MWCNT concentration were investigated in detail. Under the optimal conditions the dynamic linear range of uric acid was 2.0×10(-7) M-7.1×10(-4) M (R=0.9993) with the detection limit low to 2.3×10(-8) M. With good selectivity and sensitivity, the biosensor was successfully applied to determine the uric acid in human serum. The results of the biosensor were in good agreement with those obtained from standard method. Therefore, the presented biosensor could be a good promise for practical applications in real samples.
在本研究中，基于含二茂铁聚合物聚（乙烯基二茂铁）（PVF）、羧基化多壁碳纳米管（c-MWCNT）和明胶（GEL）修饰的玻碳电极（GCE）构建了一种新型尿酸生物传感器。尿酸酶（UOx）通过N-乙基-N'-(3-二甲氨基丙基)碳二亚胺（EDC）和N-羟基琥珀酰亚胺（NHS）化学方法共价固定在c-MWCNT/GEL/PVF/GCE上。通过扫描电子显微镜、循环伏安法和电化学阻抗谱对c-MWCNT/GEL/PVF复合材料进行了表征。详细研究了各种实验参数，如pH值、施加电位、酶负载量、PVF和c-MWCNT浓度。在最佳条件下，尿酸的动态线性范围为2.0×10(-7) M至7.1×10(-4) M（R = 0.9993），检测限低至2.3×10(-8) M。该生物传感器具有良好的选择性和灵敏度，成功应用于测定人血清中的尿酸。生物传感器的结果与标准方法获得的结果高度一致。因此，所提出的生物传感器在实际样品的实际应用中具有良好的前景。
