Kurono Sadamu, Kaneko Yuka, Matsuura Shuji, Niwayama Satomi
Laboratory of Molecular Signature Analysis, Division of Health Sciences, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan; Laboratory Chemicals Division, Wako Pure Chemical Industries, Ltd., 3-1-2 Doshomachi, Chuo-ku, Osaka, Osaka 540-8605, Japan.
Laboratory of Molecular Signature Analysis, Division of Health Sciences, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka 565-0871, Japan.
Bioorg Med Chem Lett. 2015 Mar 1;25(5):1110-6. doi: 10.1016/j.bmcl.2014.12.090. Epub 2015 Jan 7.
The combination of cysteine-specific modifiers, iodoacetanilide (IAA) and (13)C7-labeled iodoacetanilide ((13)C7-IAA), has been applied to absolute quantification of proteins. The selected reaction monitoring (SRM) with the use of nano liquid chromatography/nanoelectrospray ionization ion trap mass spectrometry (nano LC/nano-ESI-IT-MS) analysis was applied to precise quantification of three commercial proteins. Good correlation was observed between the theoretical ratios and observed ratios for all these proteins both in a simple buffer solution and in a complex protein environment. Due to efficient tagging, this method does not require separate synthesis of isotope-labeled peptides for the SRM studies. Therefore, this method is expected to be a useful tool for proteomics research.
半胱氨酸特异性修饰剂碘乙酰苯胺（IAA）和（13）C7标记的碘乙酰苯胺（（13）C7-IAA）的组合已应用于蛋白质的绝对定量。采用纳米液相色谱/纳米电喷雾电离离子阱质谱（nano LC/nano-ESI-IT-MS）分析的选择反应监测（SRM）用于三种商业蛋白质的精确定量。在简单缓冲溶液和复杂蛋白质环境中，所有这些蛋白质的理论比率和观察比率之间均观察到良好的相关性。由于标记效率高，该方法不需要为SRM研究单独合成同位素标记的肽段。因此，该方法有望成为蛋白质组学研究的有用工具。
