Kosiński S, Wieczerzak M, Janecka A, Koziołkiewicz W
Department of Physiology, Institute of Physiology and Biochemistry, School of Medicine, Lódź.
Acta Physiol Pol. 1989 Jul-Aug;40(4):440-8.
The present study is a continuation of our previous experiments on DSIP activity which have revealed that nonapeptide DSIP inhibits hippocampal electrical activity of the 4-7 c/s frequency band. The aim of the present study was to find which of the known DSIP fragments is responsible for its activity, i.e. to find the active site of the molecule. The experiments were carried out with the entire DSIP molecule and its three different fragments. The method of threshold continuous arousal pattern (TCAP) monitoring was used as the indicator of DSIP activity. It was found that the entire DSIP molecule increased TCAP, while its 1-5 fragment decreased it 1-4 and 5-9 fragments had no noticeable effect.
本研究是我们之前关于DSIP活性实验的延续，这些实验已表明九肽DSIP可抑制4 - 7赫兹频段的海马电活动。本研究的目的是找出已知的DSIP片段中哪一个对其活性起作用，即找出该分子的活性位点。实验使用了完整的DSIP分子及其三个不同片段。阈值连续唤醒模式（TCAP）监测方法被用作DSIP活性的指标。结果发现，完整的DSIP分子可增加TCAP，而其1 - 5片段可降低TCAP，1 - 4和5 - 9片段则无明显影响。
