从蜥蜴睫状神经节的杯状神经末梢记录到的突触前钙电流。

Presynaptic calcium currents recorded from calyciform nerve terminals in the lizard ciliary ganglion.

作者信息

Martin A R, Patel V, Faille L, Mallart A

机构信息

Department of Physiology, University of Colorado School of Medicine, Denver 80262.

出版信息

Neurosci Lett. 1989 Oct 23;105(1-2):14-8. doi: 10.1016/0304-3940(89)90004-9.

DOI:10.1016/0304-3940(89)90004-9

PMID:2562058

Abstract

Electron microscopic examination of ciliary ganglion from Anolis carolinensis shows large calyciform presynaptic nerve terminals ending on ganglion cells. Intracellular records were obtained from the terminals, and membrane currents were recorded with the single-electrode voltage clamp technique. After block of Na+ currents with tetrodotoxin, depolarization of the terminals produced inward currents that disappeared when extracellular Ca2+ was removed, and increased in magnitude and duration when competing outward currents were blocked by intracellular Cs+. Thus it is possible, with this preparation, to record and characterize Ca2+ currents presumably associated with neurotransmitter release.

摘要

对卡罗来纳安乐蜥睫状神经节进行电子显微镜检查，结果显示有大型花萼状突触前神经末梢终止于神经节细胞。从这些末梢获取了细胞内记录，并采用单电极电压钳技术记录了膜电流。在用河豚毒素阻断钠电流后，使这些末梢去极化会产生内向电流，当去除细胞外钙离子时该电流消失，而当细胞内铯阻断外向竞争性电流时，该电流的幅度和持续时间会增加。因此，利用这种制备方法有可能记录并表征可能与神经递质释放相关的钙电流。