利用时间聚焦对光动力疗法进行三维分辨靶向治疗。
3D-resolved targeting of photodynamic therapy using temporal focusing.
作者信息
Rowlands Christopher J, Wu Jackie, Uzel Sebastien G M, Klein Oliver, Evans Conor L, So Peter T C
机构信息
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
Department of Mechanical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
出版信息
Laser Phys Lett. 2014 Nov;11(11). doi: 10.1088/1612-2011/11/11/115605.
Abstract
A method for selectively inducing apoptosis in tumor nodules is presented, with close-to-cellular level resolution, using 3D-resolved widefield temporal focusing illumination. Treatment times on the order of seconds were achieved using Verteporfin as the photosensitizer, with doses of 30 g ml and below. Results were achieved on both 2D and 3D cell cultures, demonstrating that treatment was possible through approximately one hundred microns of dense tumor nodules.
摘要
本文提出了一种使用三维分辨宽场时间聚焦照明技术，以接近细胞水平的分辨率在肿瘤结节中选择性诱导细胞凋亡的方法。使用维替泊芬作为光敏剂，在剂量为30μg/ml及以下时，实现了以秒为单位的治疗时间。在二维和三维细胞培养中均取得了成果，表明通过约一百微米厚的致密肿瘤结节进行治疗是可行的。
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