Gopurappilly Renjitha, Bhonde Ramesh
School of Regenerative Medicine (SORM), Manipal University, Bangalore 560065, India.
School of Regenerative Medicine (SORM), Manipal University, Bangalore 560065, India.
Genomics. 2015 Apr;105(4):211-9. doi: 10.1016/j.ygeno.2015.01.003. Epub 2015 Jan 23.
We have earlier reported the generation of islet-like clusters (ILCs) from mesenchymal stromal cell (MSC)-like cells present in murine pancreas. Here we compare these ILCs to native primary islets by transcriptome screening. Genes were categorized into functional clusters and network analysis was done by Ingenuity Pathway Analysis (IPA). The fold changes for a selected panel of molecules were validated with quantitative real time PCR. A differential expression of 6516 genes (p-value ≤ 0.05, 1.5 fold change) with upregulated expression of numerous inflammatory and 'Epithelial to Mesenchymal Transition' molecules (EMT) was seen. A significant increase in the early β-cell marker expression in the ILCs indicated their progenitor status. Although not fully mature, ILCs offer certain advantages including the large number of easily inducible initiator MSCs. These 'naïve' cells may aid to devise protocols for generating functional islet equivalents. Moreover their maturation upon transplantation under local microenvironmental niche is highly possible.
我们之前报道过从小鼠胰腺中存在的间充质基质样细胞生成胰岛样簇(ILCs)。在此,我们通过转录组筛选将这些ILCs与天然原代胰岛进行比较。基因被分类到功能簇中,并通过Ingenuity Pathway Analysis(IPA)进行网络分析。通过定量实时PCR验证了一组选定分子的倍数变化。观察到6516个基因的差异表达(p值≤0.05,1.5倍变化),其中许多炎症和“上皮向间充质转化”分子(EMT)的表达上调。ILCs中早期β细胞标志物表达的显著增加表明了它们的祖细胞状态。尽管ILCs尚未完全成熟,但它们具有某些优势,包括大量易于诱导的起始间充质基质细胞(MSCs)。这些“原始”细胞可能有助于设计生成功能性胰岛等效物的方案。此外,它们在局部微环境龛下移植时高度可能成熟。