Merendino R A, Arena A, Zummo S, Mesiti M, Chillemi S, Bonina L
Institute of Medical Microbiology, University of Messina, Italy.
G Batteriol Virol Immunol. 1989 Jan-Dec;82(1-12):17-24.
Concerning the modulatory role of LPS on immunocompetent cells, the production of interferon by macrophages primed with human gamma interferon (HUIFN gamma), was studied. In particular the production of IFN from primed macrophages of patients affected by breast cancer, differentiated in presence of autologous serum or in serum from healthy donors, was compared with the IFN production from macrophages of healthy donors. The levels of endotoxin-induced IFN were enhanced by priming macrophages with HUIFN gamma. The "in vitro" treatment with Escherichia coli LPS restores the interferon production of primed macrophages, obtained from patients affected by breast cancer, differentiated in presence of serum from healthy donors. Moreover, LPS treatment of primed macrophages from patients, differentiated in autologous serum, did not influence the interferon production of these cells. Nevertheless, primed macrophages from healthy donors appeared to be more sensitive to LPS treatment in comparison with primed macrophages from patients affected by breast cancer.
关于脂多糖（LPS）对免疫活性细胞的调节作用，研究了用人类γ干扰素（HUIFNγ）预处理的巨噬细胞产生干扰素的情况。具体而言，将乳腺癌患者经自体血清或健康供体血清分化的预处理巨噬细胞产生的干扰素与健康供体巨噬细胞产生的干扰素进行了比较。用HUIFNγ预处理巨噬细胞可提高内毒素诱导的干扰素水平。用大肠杆菌LPS进行“体外”处理可恢复从乳腺癌患者获取的、在健康供体血清中分化的预处理巨噬细胞的干扰素产生。此外，对在自体血清中分化的患者预处理巨噬细胞进行LPS处理，并不影响这些细胞的干扰素产生。然而，与乳腺癌患者的预处理巨噬细胞相比，健康供体的预处理巨噬细胞似乎对LPS处理更敏感。
