Department of Biochemistry, All India Institute of Medical Sciences (AIIMS), New Delhi, India.
Department of Medical Oncology, All India Institute of Medical Sciences (AIIMS), New Delhi, India.
Clin Chim Acta. 2015 Mar 10;442:119-24. doi: 10.1016/j.cca.2015.01.012. Epub 2015 Jan 23.
Multiple myeloma (MM) represents a malignancy of B-cells characterized by proliferation of malignant plasma cells in the bone marrow (BM). Versican (VCAN), an extracellular matrix (ECM) protein, appears to be involved in multiple processes in several cancers. Identifying optimum diagnostic markers and delineating its association with disease severity might be important for controlling MM.
Expression of VCAN and its associated molecules (β-catenin, β1 integrin and FAK) were investigated in 60 subjects to evaluate their usefulness as diagnostic marker. Circulatory and molecular levels of above molecules were analyzed in their BM and Blood using ELISA, Q-PCR and western blotting along with their ROC curve analysis.
Circulatory levels of VCAN, β-catenin and FAK were significantly higher in patients with varying significance in each stage. β-Catenin and FAK intracellular levels were significantly elevated in patients. mRNA levels of all molecules were significantly higher in BMMNCs while VCAN and β-catenin also showed increase in PBMCs. Upregulation of these molecules was also observed at protein level. ROC curve analysis for VCAN showed absolute combination of sensitivity and specificity for diagnosis in serum.
Significant elevation of VCAN and its associated molecules imply their role in MM. Optimal sensitivity and specificity of VCAN might utilize its importance as potential marker for active disease.
多发性骨髓瘤（MM）是一种 B 细胞恶性肿瘤，其特征是骨髓（BM）中恶性浆细胞的增殖。神经黏附素（VCAN）是一种细胞外基质（ECM）蛋白，似乎参与了多种癌症的多个过程。确定最佳的诊断标志物并阐明其与疾病严重程度的关系，对于控制 MM 可能很重要。
在 60 名受试者中研究了 VCAN 及其相关分子（β-连环蛋白、β1 整合素和 FAK）的表达，以评估它们作为诊断标志物的有用性。使用 ELISA、Q-PCR 和 Western blot 分析了上述分子在 BM 和血液中的循环和分子水平，并进行了 ROC 曲线分析。
不同阶段患者的 VCAN、β-连环蛋白和 FAK 循环水平均显著升高，具有不同的意义。β-连环蛋白和 FAK 的细胞内水平显著升高。所有分子的 mRNA 水平在 BMMNCs 中均显著升高，而 VCAN 和 β-连环蛋白在 PBMCs 中也有升高。这些分子的表达水平也在蛋白质水平上调。VCAN 的 ROC 曲线分析显示，血清中诊断的敏感性和特异性具有绝对组合。
VCAN 及其相关分子的显著升高表明它们在 MM 中的作用。VCAN 的最佳敏感性和特异性可能利用其作为潜在的活动性疾病标志物的重要性。
