Conde Guerri B, Sinués Porta E, Arrazola Schlamilch M, Comuñas González F, Calatayud Maldonado V
Department of Morphology, University of Zaragoza, School of Medicine, Spain.
Histol Histopathol. 1989 Apr;4(2):217-22.
The effects of glia conditioned media on the survival and differentiation of embryonic neuron cultures of the Central Nervous System is described. We established glial cultures of peritumoral areas and the culture medium was changed serially and collected as glia conditioned medium (GCM). Neuron enriched cultures plated on poly-L-lysine coated Petri dishes and after 4 days in vitro, the neuronal cultures were treated for the inhibition of glial cells. We established two groups of neuronal cultures that were respectively exposed to the GCM and to conventional culture medium. We evaluated the survival and differentiation of neuronal population in each group of cultures by contrast-phase microscopy and cellular uptake of Horse Radish Peroxidase. The cell cultures exposed to GCM showed a survival during the in vitro stages and an organization and differentiation pattern of mature neurons larger than the control cultures. This fact suggests that the glial cultures produce a diffusible neurotrophic factor that influences the neuronal response in vitro.
本文描述了神经胶质细胞条件培养基对中枢神经系统胚胎神经元培养物存活和分化的影响。我们建立了肿瘤周围区域的神经胶质细胞培养物，并连续更换培养基，收集作为神经胶质细胞条件培养基（GCM）。将富含神经元的培养物接种在聚-L-赖氨酸包被的培养皿上，体外培养4天后，对神经元培养物进行处理以抑制神经胶质细胞。我们建立了两组神经元培养物，分别暴露于GCM和传统培养基。通过相差显微镜和辣根过氧化物酶的细胞摄取，评估每组培养物中神经元群体的存活和分化。暴露于GCM的细胞培养物在体外阶段显示出存活，并且成熟神经元的组织和分化模式比对照培养物更大。这一事实表明，神经胶质细胞培养物产生一种可扩散的神经营养因子，影响体外神经元反应。
