体外培养海马星形胶质细胞上α7烟碱型乙酰胆碱受体的鉴定及其对炎症介质分泌的作用。

Identification of α7 nicotinic acetylcholine receptor on hippocampal astrocytes cultured in vitro and its role on inflammatory mediator secretion.

作者信息

Wang Yan, Zhu Ning, Wang Kewan, Zhang Zhongyi, Wang Yong

机构信息

Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China ; Department of Pharmacy, the 458 Hospital of Chinese PLA, Guangzhou 510602, Guangdong Province, China.

Department of Pharmacy, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, Guangdong Province, China.

出版信息

Neural Regen Res. 2012 Aug 5;7(22):1709-14. doi: 10.3969/j.issn.1673-5374.2012.22.005.

The present study found expressions of α7 nicotinic acetylcholine receptor on hippocampal slices and hippocampal astrocytes using double immunofluorescence stainings. Expression of glial fibrillary acidic protein in the cultured hippocampal slices and hippocampal astrocytes significantly increased, and levels of macrophage inflammatory protein 1α, RANTES, interleukin-1β, interleukin-6, and tumor necrosis factor-α increased in the supernatant of cultured astrocytes following exposure to 200 nM amyloid β protein 1-42. Preconditioning of 10 μM nicotine, a nicotinic acetylcholine receptor agonist, could attenuate the influence of amyloid β protein 1-42 in inflammatory mediator secretion of cultured astrocytes. Experimental findings indicated that α7 nicotinic acetylcholine receptor was expressed on the surface of hippocampal astrocytes, and activated α7 nicotinic acetylcholine receptor was shown to inhibit inflammation induced by amyloid β protein 1-42.

本研究通过双重免疫荧光染色发现海马切片和海马星形胶质细胞上存在α7烟碱型乙酰胆碱受体的表达。培养的海马切片和海马星形胶质细胞中胶质纤维酸性蛋白的表达显著增加，并且在暴露于200 nM淀粉样β蛋白1-42后，培养的星形胶质细胞上清液中巨噬细胞炎性蛋白1α、调节激活正常T细胞表达和分泌因子、白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α的水平升高。烟碱型乙酰胆碱受体激动剂10 μM尼古丁预处理可减弱淀粉样β蛋白1-42对培养的星形胶质细胞炎性介质分泌的影响。实验结果表明，α7烟碱型乙酰胆碱受体表达于海马星形胶质细胞表面，且激活的α7烟碱型乙酰胆碱受体可抑制淀粉样β蛋白1-42诱导的炎症。