Wang Bingqi, Chen Lei, Liu Bin, Liu Zhigang, Zhang Zhixin, Pan Yuehai, Song Liangsong, Lu Laijin
Department of Hand and Foot Surgery, First Hospital of Jilin University, Changchun 130031, Jilin Province, China.
Neural Regen Res. 2012 Aug 15;7(23):1786-90. doi: 10.3969/j.issn.1673-5374.2012.23.004.
An experimental model of brachial plexus root avulsion injury of cervical dorsal C5-6 was established in adult and neonatal rats. Real-time PCR showed that the levels of brain-derived neurotrophic factor, nerve growth factor and neurotrophin-3 in adult rats increased rapidly 1 day after brachial plexus root avulsion injury, and then gradually decreased to normal levels by 21 days. In neonatal rats, levels of the three neurotrophic factors were decreased on the first day after injury, and then gradually increased from the seventh day and remained at high levels for an extended period of time. We observed that greater neural plasticity contributed to better functional recovery in neonatal rats after brachial plexus root avulsion injury compared with adult rats. Moreover, immunohistochemical staining showed that the number of bromodeoxyuridine/nestin-positive cells increased significantly in the spinal cords of the adult rats compared with neonatal rats after brachial plexus root avulsion injury. In addition, the number of bromodeoxyuridine/glial fibrillary acidic protein-positive cells in adult rats was significantly higher than in neonatal rats 14 and 35 days after brachial plexus injury. Bromodeoxyuridine/β-tubulin-positive cells were not found in either adult or neonatal rats. These results indicate that neural stem cells differentiate mainly into astrocytes after brachial plexus root avulsion injury. Furthermore, the degree of neural stem cell differentiation in neonatal rats was lower than in adult rats.
在成年和新生大鼠中建立了颈背C5-6臂丛神经根撕脱伤的实验模型。实时聚合酶链反应显示，成年大鼠臂丛神经根撕脱伤后1天，脑源性神经营养因子、神经生长因子和神经营养素-3水平迅速升高，然后在21天时逐渐降至正常水平。在新生大鼠中，损伤后第一天这三种神经营养因子的水平下降，然后从第七天开始逐渐升高，并在较长时间内保持在高水平。我们观察到，与成年大鼠相比，新生大鼠臂丛神经根撕脱伤后更大的神经可塑性有助于更好的功能恢复。此外，免疫组织化学染色显示，臂丛神经根撕脱伤后，成年大鼠脊髓中溴脱氧尿苷/巢蛋白阳性细胞的数量比新生大鼠显著增加。此外，臂丛神经损伤后14天和35天，成年大鼠中溴脱氧尿苷/胶质纤维酸性蛋白阳性细胞的数量显著高于新生大鼠。在成年和新生大鼠中均未发现溴脱氧尿苷/β-微管蛋白阳性细胞。这些结果表明，臂丛神经根撕脱伤后神经干细胞主要分化为星形胶质细胞。此外，新生大鼠神经干细胞的分化程度低于成年大鼠。
