Nicastro Giuseppe, Taylor Ian A, Ramos Andres
Division of Molecular Structure, MRC National Institute for Medical Research, London, UK.
Research Department of Structural and Molecular Biology, University College London, London, UK; Division of Molecular Structure, MRC National Institute for Medical Research, London, UK.
Curr Opin Struct Biol. 2015 Feb;30:63-70. doi: 10.1016/j.sbi.2015.01.002. Epub 2015 Jan 24.
The hnRNP K-homology (KH) domain is a single stranded nucleic acid binding domain that mediates RNA target recognition by a large group of gene regulators. The structure of the KH fold is well characterised and some initial rules for KH-RNA recognition have been drafted. However, recent findings have shown that these rules need to be revisited and have now provided a better understanding of how the domain can recognise a sequence landscape larger than previously thought as well as revealing the diversity of structural expansions to the KH domain. Finally, novel structural and functional data show how multiple KH domains act in a combinatorial fashion to both allow recognition of longer RNA motifs and remodelling of the RNA structure. These advances set the scene for a detailed molecular understanding of KH selection of the cellular targets.
不均一核糖核蛋白K同源（KH）结构域是一种单链核酸结合结构域，由一大类基因调节因子介导RNA靶标的识别。KH折叠的结构已得到充分表征，并且已经起草了一些KH-RNA识别的初步规则。然而，最近的研究结果表明，这些规则需要重新审视，现在已经对该结构域如何识别比以前认为的更大的序列格局以及揭示KH结构域的结构扩展多样性有了更好的理解。最后，新的结构和功能数据表明多个KH结构域如何以组合方式发挥作用，既能识别更长的RNA基序，又能重塑RNA结构。这些进展为详细分子理解KH对细胞靶标的选择奠定了基础。
