Balsam Joshua, Bruck Hugh Alan, Rasooly Avraham
Division of Biology, Office of Science and Engineering, FDA, 10903 New Hampshire Avenue, Silver Spring, MD, 20993, USA.
Methods Mol Biol. 2015;1256:247-58. doi: 10.1007/978-1-4939-2172-0_17.
There is a new potential to address needs for medical diagnostics in Point-of-Care (PoC) applications using mHealth (Mobile computing, medical sensors, and communications technologies for health care), a mHealth based lab test will require a LOC to perform clinical analysis. In this work, we describe the design of a simple Lab-on-a-chip (LOC) platform for mHealth medical diagnostics. The LOC utilizes a passive capillary valve with no moving parts for fluid control using channels with very low aspect ratios cross sections (i.e., channel width ≫ height) achieved through transitions in the channel geometry via that arrest capillary flow. Using a CO2 laser in raster engraving mode, we have designed and fabricated an eight-channel LOC for fluorescence signal detection fabricated by engraving and combining just two polymer layers. Each of the LOC channels is capable of mixing two reagents (e.g., enzyme and substrate) for various assays. For mHealth detection, we used a mobile CCD detector equipped with LED multispectral illumination in the red, green, blue, and white range. This technology enables the development of low-cost LOC platforms for mHealth whose fabrication is compatible with standard industrial plastic fabrication processes to enable mass production of mHealth diagnostic devices, which may broaden the use of LOCs in PoC applications, especially in global health settings.
利用移动医疗（用于医疗保健的移动计算、医疗传感器和通信技术）来满足即时医疗（PoC）应用中的医学诊断需求具有新的潜力，基于移动医疗的实验室检测将需要一个微流控芯片实验室（LOC）来进行临床分析。在这项工作中，我们描述了一种用于移动医疗医学诊断的简单微流控芯片实验室（LOC）平台的设计。该LOC利用一个无移动部件的被动毛细管阀，通过通道几何形状的转变实现极低纵横比横截面（即通道宽度≫高度）的通道来控制流体，从而阻止毛细管流动。使用处于光栅雕刻模式的二氧化碳激光器，我们设计并制造了一个用于荧光信号检测的八通道LOC，它仅通过雕刻和组合两个聚合物层制成。每个LOC通道能够混合两种试剂（例如酶和底物）以进行各种检测。对于移动医疗检测，我们使用了一个配备有红、绿、蓝和白色范围的LED多光谱照明的移动CCD探测器。这项技术能够开发用于移动医疗的低成本LOC平台，其制造与标准工业塑料制造工艺兼容，以实现移动医疗诊断设备的大规模生产，这可能会扩大LOC在即时医疗应用中的使用，特别是在全球卫生环境中。
