环孢素对部分肝切除大鼠肝脏再生的影响。

Effect of cyclosporine on liver regeneration in partial hepatectomized rats.

作者信息

Baretta Giorgio Alfredo Pedroso, Gama Filho Ozimo, Toderke Edimar Leandro, Tolazzi André Ricardo Dall'Oglio, Matias Jorge Eduardo Fouto

机构信息

Department of Surgery and Liver Transplantation, Federal University of Parana, Curitiba, PR, Brazil.

Department of Surgery and Liver Transplantation, UFPR, Curitiba, PR, Brazil.

出版信息

Acta Cir Bras. 2015 Jan;30(1):54-9. doi: 10.1590/S0102-86502015001000007.

DOI:10.1590/S0102-86502015001000007

PMID:25627271

Abstract

PURPOSE

To evaluate the influence of the cyclosporine in liver regeneration in rats submitted to an experimental model of 70% hepatectomy.

METHODS

Forty male rats were randomly divided in four subgroups (C.24h, C.7d, E.24h, E.7d), according to the drug used and the day of sacrifice (24 hours and 7 days). Cyclosporine (10 mg/Kg/day) was given to the study subgroup and 1 ml of 0.9% sodium chloride was to the control subgroup. Resection of left lateral lobe and median lobe performing 70% of liver mass. During the animals' death, KWON formula was applied. Counting of mitotic figures and percentage of positive nucleus with PCNA and Ki-67 were evaluated.

RESULTS

In the 2nd, 4th PO and death days, E.7d lose more weight than C.7d. Regarding to the KWON formula, the C.7d regenerated more than the C.24h and the same with the E.7d. Comparing between the groups, only E7d subgroup was statistically significant compared with C.7d, showing the stimulating effect of cyclosporine in liver regeneration. Immunohistochemestry had significant results between the study subgroups. The mitotic index revealed statistical differences in the control subgroups.

CONCLUSION

Cyclosporine, in spite of being an immunosuppressive drug, has a positive effect in liver regeneration, although reduce the animal's body weight.

摘要

目的

在大鼠70%肝切除实验模型中，评估环孢素对肝脏再生的影响。

方法

根据所用药物及处死时间（24小时和7天），将40只雄性大鼠随机分为四个亚组（C.24h、C.7d、E.24h、E.7d）。给研究亚组注射环孢素（10毫克/千克/天），给对照亚组注射1毫升0.9%氯化钠溶液。切除左外叶和中叶，切除肝脏重量的70%。在动物处死时，应用权公式。评估有丝分裂指数以及PCNA和Ki-67阳性细胞核的百分比。

结果

在术后第2天、第4天和处死日，E.7d组比C.7d组体重减轻更多。关于权公式，C.7d组比C.24h组再生更多，E.7d组也是如此。在各亚组之间进行比较，只有E7d亚组与C.7d组相比有统计学意义，表明环孢素对肝脏再生有刺激作用。免疫组织化学在研究亚组之间有显著结果。有丝分裂指数在对照亚组中有统计学差异。