Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921, Korea.
Center for Fungal Pathogenesis, Seoul National University, Seoul, 151-921, Korea.
Environ Microbiol. 2015 Jun;17(6):2048-63. doi: 10.1111/1462-2920.12757. Epub 2015 Mar 4.
Sodium is an abundant cation required for protein function and maintenance of cellular osmotic homeostasis. High concentrations of sodium are toxic, and fungi have evolved efficient sodium efflux systems. In this study, we characterized a novel sodium tolerance mechanism in the plant pathogen Fusarium graminearum. Fusarium graminearum sodium sensitive 1 (Fss1) is a nuclear transcription factor with a Zn(II)2 Cys6 fungal-type DNA-binding domain required for sodium tolerance. RNA-seq and genetic studies revealed that a P-type ATPase pump, exitus natru (Latin: exit sodium) 1 (FgEna5), mediates the phenotypic defects of FSS1 mutants. A homologue of PACC (PAC1) was required for FgEna5-dependent sodium and lithium tolerance independent of Fss1. The results of this study revealed that F. graminearum has a distinct and novel pathway for sodium tolerance not present in other model fungi.
钠是一种丰富的阳离子，对于蛋白质功能和细胞渗透压平衡的维持至关重要。高浓度的钠是有毒的，真菌已经进化出了有效的钠离子外排系统。在这项研究中，我们描述了植物病原体禾谷镰刀菌中一种新的耐钠机制。禾谷镰刀菌钠敏感 1 （Fss1）是一种核转录因子，具有 Zn(II)2 Cys6 真菌型 DNA 结合域，对于耐钠性是必需的。RNA-seq 和遗传研究表明，一种 P 型 ATP 酶泵，exitus natru （拉丁语：排出钠）1 （FgEna5），介导 FSS1 突变体的表型缺陷。PACC（PAC1）的同源物对于 FgEna5 依赖性的钠和锂耐受是必需的，与 Fss1 无关。这项研究的结果表明，禾谷镰刀菌具有一种独特而新颖的耐钠途径，而其他模式真菌中并不存在。
