Lewis D J
Food and Environment Research Agency, Sand Hutton, York YO41 1LZ, UK.
Analyst. 2015 Mar 7;140(5):1624-8. doi: 10.1039/c4an01979b. Epub 2015 Jan 28.
Hydrodynamic chromatography - inductively coupled plasma mass spectrometry (HDC-ICP-MS) is a technique that is widely used in the size-characterisation of nanoparticles. In this work, a system was modified to facilitate the injection of NIST-traceable standards into the post-column effluent, which then allowed the response from an eluting nanoparticle to be quantified against the response from the post-column standards. Combining the simultaneously acquired particle sizing data and mass concentration data allowed accurate quantification of the particle number concentration to be made in a single analytical run. This unique single-method approach was successfully validated against a nanoparticle system which had previously been characterised in a number of recent peer-reviewed publications. In addition to this, its robustness was assessed using extracts from a study investigating the fate of nanoparticles in sewage sludge, and found to provide much improved data compared to what might have been achieved using an external calibration approach. With in-vial limits of detection of 2 and 10 ng ml(-1) for titanium and silver respectively, it is insufficient for use with environmental waters, but is foreseen as being useful in screening nanoparticle production processes, or in the characterisation of higher concentration materials. As this instrumental configuration is likely to be of use to researchers involved in the general area of quantitative trace element speciation, detailed description of construction of the interface is given as ESI.
流体动力学色谱-电感耦合等离子体质谱法（HDC-ICP-MS）是一种广泛用于纳米颗粒尺寸表征的技术。在这项工作中，对一个系统进行了改进，以便将可溯源至美国国家标准与技术研究院（NIST）的标准物质注入柱后流出物中，从而能够根据柱后标准物质的响应来定量洗脱纳米颗粒的响应。结合同时获取的颗粒尺寸数据和质量浓度数据，可在一次分析运行中准确地对颗粒数浓度进行定量。这种独特的单一方法已针对一个纳米颗粒系统成功进行了验证，该系统在近期的一些同行评审出版物中已有描述。除此之外，使用一项研究污水污泥中纳米颗粒归宿的研究提取物评估了其稳健性，结果发现与使用外部校准方法相比，它能提供质量有很大提高的数据。钛和银在瓶内的检测限分别为2和10 ng ml⁻¹，虽然它不适用于环境水样，但预计可用于筛选纳米颗粒生产工艺或表征高浓度材料。由于这种仪器配置可能对从事定量微量元素形态分析一般领域的研究人员有用，因此在电子补充材料（ESI）中给出了接口构建的详细描述。
