Fainerman V B, Aksenenko E V, Lylyk S V, Lotfi M, Miller R
Medical University Donetsk , Donetsk 83003, Ukraine.
J Phys Chem B. 2015 Mar 5;119(9):3768-75. doi: 10.1021/acs.jpcb.5b00136. Epub 2015 Feb 19.
The influence of the addition of the nonionic surfactants C12DMPO, C14DMPO, C10OH, and C10EO5 at concentrations between 10(-5) and 10(-1) mmol/L to solutions of β-casein (BCS) and β-lactoglobulin (BLG) at a fixed concentration of 10(-5) mmol/L on the dilational surface rheology is studied. A maximum in the viscoelasticity modulus |E| occurs at very low surfactant concentrations (10(-4) to 10(-3) mmol/L) for mixtures of BCS with C12DMPO and C14DMPO and for mixtures of BLG with C10EO5, while for mixture of BCS with C10EO5 the value of |E| only slightly increased. The |E| values calculated with a recently developed model, which assumes changes in the interfacial molar area of the protein molecules due to the interaction with the surfactants, are in satisfactory agreement with experimental data. A linear dependence exists between the ratio of the maximum modulus for the mixture to the modulus of the single protein solution and the coefficient reflecting the influence of the surfactants on the adsorption activity of the protein.
研究了在浓度为10(-5) mmol/L的β-酪蛋白（BCS）和β-乳球蛋白（BLG）溶液中添加浓度在10(-5)至10(-1) mmol/L之间的非离子表面活性剂C12DMPO、C14DMPO、C10OH和C10EO5对扩张表面流变学的影响。对于BCS与C12DMPO和C14DMPO的混合物以及BLG与C10EO5的混合物，在非常低的表面活性剂浓度（10(-4)至10(-3) mmol/L）下，粘弹性模量|E|出现最大值，而对于BCS与C10EO5的混合物，|E|值仅略有增加。用最近开发的模型计算的|E|值，该模型假设由于与表面活性剂的相互作用蛋白质分子的界面摩尔面积发生变化，与实验数据吻合良好。混合物的最大模量与单一蛋白质溶液的模量之比与反映表面活性剂对蛋白质吸附活性影响的系数之间存在线性关系。
