几种聚环氧乙烷-聚环氧丙烷两亲物对bax、bcl-2和hTERT mRNA的影响：深入了解这些聚合物辅料诱导肝癌细胞凋亡和细胞永生化的机制

Effect of several PEO-PPO amphiphiles on bax, bcl-2, and hTERT mRNAs: An insight into apoptosis and cell immortalization induced in hepatoma cells by these polymeric excipients.

作者信息

Castronuovo Cynthia Celeste, Cuestas María Luján, Oubiña José Raúl, Mathet Verónica Lidia

机构信息

Instituto de Investigaciones en Microbiología y Parasitología Médica, UBA-CONICET, Facultad de Medicina, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.

CONICET, Ciudad Autónoma de Buenos Aires, Argentina.

出版信息

Biotechnol Appl Biochem. 2016 Mar-Apr;63(2):273-80. doi: 10.1002/bab.1352. Epub 2015 May 14.

DOI:10.1002/bab.1352

PMID:25630439

Abstract

UNLABELLED

Recent data have shown that synthetic polymers and nanomaterials display phenotypic effects in cells and signal transduction mechanisms involved in inflammation, differentiation, proliferation, and apoptosis.

AIM

This article aims to investigate the effect of poly(ethylene oxide)-poly(propylene oxide) (PEO-PPO) block copolymers with a wide range of biomedical and pharmaceutical applications on apoptosis and/or cell immortalization, by flow cytometry and multiplex RT-PCR for bax, bcl-2, and human telomerase reverse transcriptase (hTERT).

RESULTS

PEO-PPO amphiphiles upregulated bax and hTERT and induced apoptosis of two human hepatoma cell lines.

CONCLUSIONS

PEO-PPO block copolymers-considered safe for human use-can drastically alter gene expression profiles of genes related to apoptosis/cell proliferation.

摘要