Daryabari H, Akhlaghi A, Zamiri M J, Pirsaraei Z Ansari, Mianji G Rahimi, Deldar H, Eghbalian A N
Department of Animal Science, College of Agriculture, Shiraz University, Shiraz 71441-65186, Iran.
Department of Animal Science, College of Agriculture, Shiraz University, Shiraz 71441-65186, Iran
Poult Sci. 2015 Feb;94(2):289-95. doi: 10.3382/ps/peu073. Epub 2015 Jan 27.
Probable involvement of avidin and avidin-related protein-2 (AVR2) in sperm viability in the sperm storage tubules of turkeys has been suggested. The high affinity of biotin to avidin and its analogs is also well documented. The present study aimed to determine the effect of oral biotin on reproductive performance and oviductal mRNA expression of avidin and AVR2 in 2 broiler hen lines with different fertility rates. Low-fertility (line B) and high-fertility (line D) hens (n=144) were randomly allotted to receive 0 (T0), 0.30 (T1), or 0.45 (T2) mg/L biotin in drinking water from 30 through 33 wk of age. The reproductive performance of the hens was evaluated using artificial insemination. At the end of the treatment period, 24 hens per line were killed to assay the expression of avidin and AVR2 in the uterovaginal junction. Supplementary biotin increased egg production from 73.5% for T0 to 87.8% for T2. Hens administered with biotin in line B, but not in line D, showed an increase (8.4%) in fertility rate. Hatchability, chick quality, and overall embryonic mortality were not different among the experimental groups. Real-time PCR data showed that both avidin (P=0.0013) and AVR2 (P<0.0001) expressions were influenced by a biotin×line interaction effect, where low-fertility line B hens receiving the high biotin level recorded respectively a 3.9 and 15.3% increase in avidin and AVR2 mRNA expression, although biotin did not affect these traits in line D hens. Control hens in line D had a dramatically higher AVR2 expression record (7.4-fold) compared with the control hens in line B. The correlation coefficients of fertility rate and avidin expression were 0.73 and 0.66 in lines B and D, respectively. However, the correlation of fertility and AVR2 (r=0.65) was significant for line D hens only. Overall, fertility rate and oviductal expression of avidin and AVR2 were dichotomously affected by oral biotin in low- and high-fertility line hens, where only low-fertility birds showed improvements in these attributes.
有人提出抗生物素蛋白和抗生物素蛋白相关蛋白2（AVR2）可能参与火鸡精子储存管中精子的活力。生物素与抗生物素蛋白及其类似物的高亲和力也有充分的文献记载。本研究旨在确定口服生物素对两个具有不同受精率的肉鸡品系生殖性能以及抗生物素蛋白和AVR2在输卵管中的mRNA表达的影响。低受精率（B系）和高受精率（D系）母鸡（n = 144）在30至33周龄时被随机分配，饮用含0（T0）、0.30（T1）或0.45（T2）mg/L生物素的水。通过人工授精评估母鸡的生殖性能。在治疗期结束时，每个品系处死24只母鸡，以检测子宫阴道连接处抗生物素蛋白和AVR2的表达。补充生物素使产蛋率从T0组的73.5%提高到T2组的87.8%。B系中给予生物素的母鸡受精率提高（8.4%），而D系中给予生物素的母鸡受精率未提高。各实验组之间的孵化率、雏鸡质量和总体胚胎死亡率没有差异。实时PCR数据显示，抗生物素蛋白（P = 0.0013）和AVR2（P < 0.0001）的表达均受生物素×品系交互作用的影响，其中接受高生物素水平的低受精率B系母鸡抗生物素蛋白和AVR2的mRNA表达分别增加了3.9%和15.3%，尽管生物素对D系母鸡的这些性状没有影响。D系的对照母鸡与B系的对照母鸡相比，AVR2表达记录显著更高（7.4倍）。B系和D系中受精率与抗生物素蛋白表达的相关系数分别为0.73和0.66。然而，受精率与AVR2的相关性（r = 0.65）仅在D系母鸡中显著。总体而言，口服生物素对低受精率和高受精率品系母鸡的受精率以及输卵管中抗生物素蛋白和AVR2的表达有不同影响，只有低受精率的母鸡在这些方面有所改善。
