Department of Dermatology, Qilu Hospital, Shandong University, No 107 Wenhua Xi Road, Jinan, 250012, People's Republic of China.
Inflammation. 2015;38(3):1003-11. doi: 10.1007/s10753-014-0064-2.
Corticotropin-releasing hormone (CRH) and CRH receptors (CRH-Rs) are expressed in the skin; CRH-R1 is the predominant receptor. Whether the CRH/CRH-R1 system plays a role in psoriasis has not yet been assessed. Immunohistochemistry, real-time RT-PCR, ELISA assay, and Western blot analysis were used to investigate the expression of CRH/CRH-R1 in patients with chronic plaque psoriasis and that of IL-18 in CRH-treated HaCaT cells. CRH and CRH-R1 were downregulated in patients with chronic plaque psoriasis. In vitro, CRH attenuated the expression of IL-18 by a mitogen-activated protein kinase signaling pathway through CRH-R1 in HaCaT cells. Thus, an aberrant cutaneous CRH/CRH-R1 system exists in lesions from chronic plaque psoriasis which might play a role in psoriasis and offers further evidence for the study of CRH in the skin.
促肾上腺皮质激素释放激素（CRH）及其受体（CRH-Rs）在皮肤中表达；CRH-R1 是主要的受体。CRH/CRH-R1 系统是否在银屑病中发挥作用尚未得到评估。本研究采用免疫组织化学、实时 RT-PCR、ELISA 检测和 Western blot 分析方法，研究慢性斑块状银屑病患者的 CRH/CRH-R1 表达和 CRH 处理 HaCaT 细胞后白细胞介素-18（IL-18）的表达。结果发现，慢性斑块状银屑病患者的 CRH 和 CRH-R1 表达下调。体外研究显示，CRH 通过 CRH-R1 对 HaCaT 细胞中的丝裂原活化蛋白激酶信号通路，减弱了 IL-18 的表达。因此，慢性斑块状银屑病皮损中存在异常的皮肤 CRH/CRH-R1 系统，可能在银屑病中发挥作用，并为皮肤中 CRH 的研究提供了进一步的证据。
