Wang Wei, Esbensen Ying, Scheffler Katja, Eide Lars
Department of Medical Biochemistry, Institute of Clinical Medicine, Rikshospitalet, University of Oslo, Sognsvannsveien 20, 0027, Oslo, Norway.
Methods Mol Biol. 2015;1264:97-106. doi: 10.1007/978-1-4939-2257-4_10.
This chapter describes the use of real-time qPCR to analyze the integrity of mitochondrial nucleic acids quantitatively. The method has low material requirement, is low cost, and can detect modifications with high resolution. The method is specifically designed for mitochondrial RNA and DNA, but can be easily transferred to other high-copy number cases. This procedure describes analyses of brain nucleic acids, but other tissues or cells can be analyzed similarly.
本章介绍了使用实时定量PCR来定量分析线粒体核酸的完整性。该方法对材料的要求低，成本低，并且能够以高分辨率检测修饰情况。该方法是专门为线粒体RNA和DNA设计的，但可以很容易地应用于其他高拷贝数的情况。本实验步骤描述了对脑核酸的分析，但其他组织或细胞也可以用类似的方法进行分析。
