Wang Geng, Shimada Eriko, Nili Mahta, Koehler Carla M, Teitell Michael A
School of Life Sciences, Tsinghua University, Beijing, 100084, China.
Methods Mol Biol. 2015;1264:107-16. doi: 10.1007/978-1-4939-2257-4_11.
The import of a modest number of nucleus-encoded RNAs into mitochondria has been reported in species ranging from yeast to human. With the advent of high-throughput RNA sequencing, additional nucleus-encoded mitochondrial RNAs are being identified. Confirming the mitochondrial localization of candidate RNAs of interest (e.g., small noncoding RNAs, miRNAs, tRNAs, and possibly lncRNAs and viral RNAs) and understanding their function within the mitochondrion is assisted by in vitro and in vivo import assay systems. Here we describe these two systems for studying mitochondrial RNA import, processing, and functions.
从酵母到人类等物种中，均有关于少量细胞核编码的RNA导入线粒体的报道。随着高通量RNA测序技术的出现，更多细胞核编码的线粒体RNA正在被鉴定出来。体外和体内导入分析系统有助于确认感兴趣的候选RNA（如小非编码RNA、miRNA、tRNA，可能还有lncRNA和病毒RNA）的线粒体定位，并了解它们在线粒体内的功能。在此，我们描述这两种用于研究线粒体RNA导入、加工及功能的系统。
