Hynes James, Carey Conn
Luxcel Biosciences Ltd., BioInnovation Centre, UCC, Western Gateway Building, Office 204, Cork, Ireland,
Methods Mol Biol. 2015;1264:203-17. doi: 10.1007/978-1-4939-2257-4_19.
Knowledge of in situ oxygenation of cells in 2D and 3D cultures offers important insights into the impact of oxygen on cellular function. Here we outline how such measurements can be performed in 2D cultures of adherent cells and also in cells cultured on 3D scaffolds. Measurements are performed on conventional time-resolved fluorescence plate readers using the intracellular oxygen-sensitive probe MitoXpress(®)-Intra. We also illustrate how the impact of drug treatment on cell oxygenation can be assessed and how the link between oxygenation and glycolytic metabolism can be examined.
了解二维和三维培养中细胞的原位氧合情况，有助于深入了解氧气对细胞功能的影响。在此，我们概述了如何在贴壁细胞的二维培养以及三维支架上培养的细胞中进行此类测量。使用细胞内氧敏感探针MitoXpress(®)-Intra在传统的时间分辨荧光酶标仪上进行测量。我们还举例说明了如何评估药物治疗对细胞氧合的影响，以及如何检测氧合与糖酵解代谢之间的联系。
