Patel Neeraj K, Bhutani Kamlesh K
Nat Prod Commun. 2014 Dec;9(12):1749-50.
In the continuous search for new antiinflammatory agents from natural products, dichloromethane (DCM), ethyl acetate (EtOAc) and methanol (MeOH) extracts of Ipomea fistulosa leaves were evaluated for inhibition of production of nitric oxide (NO), interleukin 1beta (IL-1β) and tumor necrosis factor alpha (TNF-α) in lipopolysaccharide (LPS) stimulated J774A.1 cells. Among the tested extracts, the ethyl acetate (EtOAc) extract was found to be most active and activity based fractionation of this extract by column chromatography led to the identification of seven compounds for the first time from this plant. Furthermore, 3,4-dimethoxy cinnamic acid (1) exhibited two folds more potent inhibition of LPS-induced NO production (IC50 = 10.7 μg/mL) as compared with the standard, L-NAME (IC50 = 19.8 μg/mL). The present study supports the use of Ipomea fistulosa leaves for the treatment of inflammation.
在持续不断地从天然产物中寻找新型抗炎剂的过程中，对掌叶牵牛叶子的二氯甲烷（DCM）、乙酸乙酯（EtOAc）和甲醇（MeOH）提取物进行了评估，以检测其对脂多糖（LPS）刺激的J774A.1细胞中一氧化氮（NO）、白细胞介素1β（IL-1β）和肿瘤坏死因子α（TNF-α）产生的抑制作用。在测试的提取物中，发现乙酸乙酯（EtOAc）提取物活性最强，通过柱色谱对该提取物进行基于活性的分级分离，首次从该植物中鉴定出七种化合物。此外，与标准品L-NAME（IC50 = 19.8 μg/mL）相比，3,4-二甲氧基肉桂酸（1）对LPS诱导的NO产生的抑制作用强两倍（IC50 = 10.7 μg/mL）。本研究支持将掌叶牵牛叶子用于治疗炎症。
