Bizukojc Marcin, Ledakowicz Stanislaw
Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering, Lodz University of Technology, ul. Wolczanska 213, 90-924, Lodz, Poland,
Adv Biochem Eng Biotechnol. 2015;149:133-70. doi: 10.1007/10_2014_302.
The aim of this work is to review bioprocess engineering aspects of lovastatin (antihypercholesterolemia drug) production by Aspergillus terreus in the submerged culture in the bioreactors of various scale presented in the scientific literature since the nineties of the twentieth century. The key factor influencing the cultivation of any filamentous species is fungal morphology and that is why this aspect was treated as the starting point for further considerations. Fungal morphology is known to have an impact on the following issues connected with the cultivation of A. terreus reviewed in this article. These are broth viscosity in conjunction with non-Newtonian behaviour of the cultivation broths, and multistage oxygen transfer processes: from gas phase (air) to liquid phase (broth) and diffusion in the fungal agglomerates. The latest achievements concerning the controlling A. terreus morphology during lovastatin biosynthesis with the use of morphological engineering techniques were also reviewed. Last but not least, some attention was paid to the type of a bioreactor, its operational mode and kinetic modelling of lovastatin production by A. terreus.
这项工作的目的是回顾自20世纪90年代以来科学文献中所呈现的，在不同规模生物反应器的深层培养中，土曲霉生产洛伐他汀（抗高胆固醇血症药物）的生物过程工程方面。影响任何丝状菌培养的关键因素是真菌形态，这就是为什么将这一方面作为进一步考虑的起点。众所周知，真菌形态会对本文所回顾的与土曲霉培养相关的以下问题产生影响。这些问题包括发酵液粘度以及发酵液的非牛顿行为，还有多级氧气传递过程：从气相（空气）到液相（发酵液）以及在真菌聚集体中的扩散。还回顾了利用形态工程技术在洛伐他汀生物合成过程中控制土曲霉形态的最新成果。最后但同样重要的是，对生物反应器的类型、其操作模式以及土曲霉生产洛伐他汀的动力学建模也给予了一定关注。
