Kluckova Katarina, Dong Lan-Feng, Bajzikova Martina, Rohlena Jakub, Neuzil Jiri
Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Methods Mol Biol. 2015;1265:181-94. doi: 10.1007/978-1-4939-2288-8_14.
Respiration is one of the major functions of mitochondria, whereby these vital organelles use oxygen to produce energy. Many agents that may be of potential clinical relevance act by targeting mitochondria, where they may suppress mitochondrial respiration. It is therefore important to evaluate this process and understand how this is modulated by small molecules. Here, we describe the general methodology to assess respiration in cultured cells, followed by the evaluation of the effect of one anticancer agent targeted to mitochondria on this process, and also how to assess this in tumor tissue.
呼吸作用是线粒体的主要功能之一，通过这些重要的细胞器利用氧气来产生能量。许多可能具有潜在临床意义的药物通过靶向线粒体发挥作用，在那里它们可能抑制线粒体呼吸。因此，评估这一过程并了解小分子如何调节它很重要。在这里，我们描述了评估培养细胞中呼吸作用的一般方法，随后评估一种靶向线粒体的抗癌药物对这一过程的影响，以及如何在肿瘤组织中进行评估。
