Cui Zhifeng, Gao Nana, Wang Qian, Ren Yun, Wang Kun, Zhu Tingheng
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou, 310032, People's Republic of China.
Curr Genet. 2015 Nov;61(4):545-53. doi: 10.1007/s00294-015-0474-1. Epub 2015 Jan 30.
Monocarboxylate transporters have a central role in mammalian metabolism, but rarely reported in phytopathogenic fungi. In this study, a putative monocarboxylate transporter gene in Botrytis cinerea [B. cinerea MctA (BcMctA)] was identified in the research of a B. cinerea transfer DNA (T-DNA) insertional mutant (74). Disruption of the gene decreased the growth rate on the medium with monocarboxylate (acetate or pyruvate) as the sole carbon sources, but not affected on lactate. The pyruvate contents in BcmctA deletion mutants decreased about 35 % compared with the wild strain. Besides, the conidial yield was increased about two times in BcmctA disruption mutant. The pathogenicity assay indicated that disruption of BcmctA significantly reduced the virulence of B. cinerea on cucumber and tomato leaves. Our results demonstrated that BcMctA is related to pyruvate uptake and pathogenicity of B. cinerea on cucumber and tomato leaves.
单羧酸转运蛋白在哺乳动物新陈代谢中起核心作用，但在植物病原真菌中鲜有报道。在本研究中，在灰葡萄孢转座子T-DNA插入突变体（74）的研究中鉴定出了灰葡萄孢中一个假定的单羧酸转运蛋白基因[灰葡萄孢MctA（BcMctA）]。该基因的破坏降低了以单羧酸（乙酸或丙酮酸）作为唯一碳源的培养基上的生长速率，但对乳酸没有影响。与野生菌株相比，BcmctA缺失突变体中的丙酮酸含量降低了约35%。此外，BcmctA破坏突变体中的分生孢子产量增加了约两倍。致病性测定表明，BcmctA的破坏显著降低了灰葡萄孢对黄瓜和番茄叶片的毒力。我们的结果表明，BcMctA与丙酮酸摄取以及灰葡萄孢对黄瓜和番茄叶片的致病性有关。
