Guo Rui, Chai Linlin, Chen Liang, Chen Wenguang, Ge Liangpeng, Li Xiaoge, Li Hongli, Li Shirong, Cao Chuan
Department of Plastic and Reconstructive Surgery, Southwestern Hospital, Third Military Medical University, Chongqing, 400038, China.
In Vitro Cell Dev Biol Anim. 2015 Jun;51(6):578-85. doi: 10.1007/s11626-014-9862-y. Epub 2015 Jan 31.
Epidermal stem cells could contribute to skin repair through the migration of cells from the neighboring uninjured epidermis, infundibulum, hair follicle, or sebaceous gland. However, little is known about the factors responsible for the complex biological processes in wound healing. Herein, we will show that the attracting chemokine, SDF-1/CXCR4, is a major regulator involved in the migration of epidermal stem cells during wound repair. We found that the SDF-1 levels were markedly increased at the wound margins following injury and CXCR4 expressed in epidermal stem cells and proliferating epithelial cells. Blocking the SDF-1/CXCR4 axis resulted in a significant reduction in epidermal stem cell migration toward SDF-1 in vitro and delayed wound healing in vivo, while an SDF-1 treatment enhanced epidermal stem cell migration and proliferation and accelerated wound healing. These results provide direct evidence that SDF-1 promotes epidermal stem cell migration, accelerates skin regeneration, and makes the development of new regenerative therapeutic strategies for wound healing possible.
表皮干细胞可通过邻近未受伤表皮、毛囊漏斗部、毛囊或皮脂腺的细胞迁移来促进皮肤修复。然而，对于伤口愈合过程中负责复杂生物学过程的因素，我们知之甚少。在此，我们将表明，趋化因子SDF-1/CXCR4是伤口修复过程中参与表皮干细胞迁移的主要调节因子。我们发现，损伤后伤口边缘的SDF-1水平显著升高，且CXCR4在表皮干细胞和增殖的上皮细胞中表达。阻断SDF-1/CXCR4轴会导致体外表皮干细胞向SDF-1迁移显著减少，体内伤口愈合延迟，而SDF-1处理可增强表皮干细胞迁移和增殖并加速伤口愈合。这些结果提供了直接证据，表明SDF-1促进表皮干细胞迁移，加速皮肤再生，并使开发新的伤口愈合再生治疗策略成为可能。
