Matsunaga H, Iguchi A, Yatomi A, Uemura K, Miura H, Gotoh M, Mano T, Sakamoto N
Third Department of Internal Medicine, Nagoya University School of Medicine, Japan.
Endocrinology. 1989 Mar;124(3):1259-64. doi: 10.1210/endo-124-3-1259.
We examined the relative contributions of hormones and nervous system to the total 2-deoxy-D-glucose (2-DG)-induced central nervous system-mediated hyperglycemia. 2-DG was injected into the third cerebral ventricle in the following four groups of rats, and hepatic venous plasma glucose, immunoreactive glucagon, immunoreactive insulin, epinephrine, and norepinephrine were measured: 1) intact rats; 2) intact rats receiving somatostatin with insulin infusion through the femoral vein to inhibit glucagon secretion and maintain the basal insulin level; 3) bilateral adrenalectomized (ADX) rats to prevent epinephrine secretion; and 4) ADX rats receiving somatostatin with insulin infusion. Comparing areas under glucose curves among the intact rats, those receiving somatostatin with insulin infusion, ADX rats, and ADX rats receiving somatostatin with insulin infusion, the area under the glucose curve was intact rats greater than intact rats receiving somatostatin with insulin infusion greater than ADX rats receiving somatostatin with insulin infusion greater than ADX rats. These results suggest that there are three distinct sympathetic nervous system responses to 2-DG-induced central nervous system-mediated hyperglycemia. 2-DG-induced hyperglycemia is not dependent on only one of those three systems, it is dependent on all of them. The relative potency of the factors to 2-DG-induced hyperglycemia increases in the following order: direct neural innervation of liver (including suppressive epinephrine action on insulin secretion), glucagon, and direct epinephrine action on liver.
我们研究了激素和神经系统对2-脱氧-D-葡萄糖（2-DG）诱导的中枢神经系统介导的高血糖症的相对贡献。将2-DG注入以下四组大鼠的第三脑室，并测量肝静脉血浆葡萄糖、免疫反应性胰高血糖素、免疫反应性胰岛素、肾上腺素和去甲肾上腺素：1）完整大鼠；2）通过股静脉输注胰岛素并接受生长抑素的完整大鼠，以抑制胰高血糖素分泌并维持基础胰岛素水平；3）双侧肾上腺切除（ADX）大鼠以防止肾上腺素分泌；4）接受生长抑素并输注胰岛素的ADX大鼠。比较完整大鼠、接受生长抑素并输注胰岛素的大鼠、ADX大鼠以及接受生长抑素并输注胰岛素的ADX大鼠的葡萄糖曲线下面积，葡萄糖曲线下面积的顺序为：完整大鼠大于接受生长抑素并输注胰岛素的完整大鼠大于接受生长抑素并输注胰岛素的ADX大鼠大于ADX大鼠。这些结果表明，对于2-DG诱导的中枢神经系统介导的高血糖症存在三种不同的交感神经系统反应。2-DG诱导的高血糖症并非仅依赖于这三个系统中的一个，而是依赖于所有这些系统。这些因素对2-DG诱导的高血糖症的相对效力按以下顺序增加：肝脏的直接神经支配（包括肾上腺素对胰岛素分泌的抑制作用）、胰高血糖素以及肾上腺素对肝脏的直接作用。
