Ploemacher R E, Brons R H
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Exp Hematol. 1989 Mar;17(3):263-6.
We have studied the in vivo spleen colony-forming ability and marrow repopulating ability of murine bone marrow cells differing in mitochondrial activity. Following centrifugal elutriation the cells were sorted on the basis of rhodamine-123 fluorescence intensity within a predetermined light scatter window. It is shown that a class of hemopoietic stem cells exists that differs from the majority of day-12 spleen colony-forming units (CFU-S) in that it has low mitochondrial activity and a high capacity to generate in time new day-12 CFU-S and cells that rescue recipients from radiation-inflicted death. These data add direct evidence for the identity of pre-CFU-S and CFU-S by physical separation.
我们研究了线粒体活性不同的小鼠骨髓细胞的体内脾集落形成能力和骨髓重建能力。通过离心淘洗后，在预定的光散射窗口内，根据罗丹明-123荧光强度对细胞进行分选。结果表明，存在一类造血干细胞，它与大多数第12天的脾集落形成单位（CFU-S）不同，其线粒体活性低，能够及时产生新的第12天CFU-S以及能使受辐照受体免于死亡的细胞。这些数据通过物理分离为前CFU-S和CFU-S的一致性提供了直接证据。
