Sharifian S, Kamrani E, Safaie M, Sharifian S
Hormozgan University, Faculty of Science, Bandar abbas, Iran.
Hormozgan University, Faculty of Science, Bandar abbas, Iran.
Tissue Cell. 2015 Apr;47(2):213-20. doi: 10.1016/j.tice.2014.11.006. Epub 2014 Dec 18.
In this study, the reproductive biology of female freshwater crab Sodhiana iranica, oogenesis and ovarian development were described. An H-shaped ovary consisting of a pair of long ovarian sacs connected by a narrow bridge tube was located in the cephalothorax on the dorsal side of the stomach. Females at different stages of ovarian development were anesthetized and their ovaries were removed, photographed, fixed, and processed for histological examination. Based on the light microscopic observations of cells' sizes, chromatin patterns, and amount of lipid vesicles, the female germ cells could be classified into seven different stages: (1) oogonia (Oog), (2) primary oocytes (pOc), (3) early previtellogenic oocyte (Oc1), (4) late previtellogenic oocyte(Oc2), (5) early vitellogenic oocyte (Oc3), (6) late vitellogenic oocyte (Oc4), and (7) mature oocyte (mOc). Oog are small oval-shaped cells with irregular-shaped nuclei. Oog undergo first meiotic division to become primary oocytes. The primary oocytes are small oval-shaped cells with large nuclei. The secondary oocytes derived from 2nd meiosis and comprise five steps. Four ovarian development stages were found for females based on the number and types of oocytes present in each stage: spent I (Spent), II (Proliferative) and III (Premature) and stage IV (Mature). The ovaries, macroscopically, varied in size and color during each developmental stage and, microscopically, the ovarian stages differed in proportion oogonia, and the secondary oocytes. During ovarian stage I, ovary contains primarily oogonia, primary oocytes and Oc1. In stage II, contains mainly Oc1, Oc2, and Oc3, while in stage III the predominant cells are Oc4. Mature oocytes appear synchronously in stage IV.
在本研究中，描述了雌性淡水蟹伊朗索氏蟹的生殖生物学、卵子发生和卵巢发育。一个呈H形的卵巢由一对通过狭窄桥管相连的长卵巢囊组成，位于头胸部胃的背侧。对处于不同卵巢发育阶段的雌性进行麻醉，然后摘除其卵巢，拍照、固定并进行组织学检查。基于对细胞大小、染色质模式和脂质小泡数量的光学显微镜观察，雌性生殖细胞可分为七个不同阶段：（1）卵原细胞（Oog），（2）初级卵母细胞（pOc），（3）前卵黄发生早期卵母细胞（Oc1），（4）前卵黄发生晚期卵母细胞（Oc2），（5）卵黄发生早期卵母细胞（Oc3），（6）卵黄发生晚期卵母细胞（Oc4），以及（7）成熟卵母细胞（mOc）。卵原细胞是小的椭圆形细胞，细胞核形状不规则。卵原细胞进行第一次减数分裂成为初级卵母细胞。初级卵母细胞是小的椭圆形细胞，细胞核较大。次级卵母细胞由第二次减数分裂产生，包括五个步骤。根据每个阶段存在的卵母细胞数量和类型，发现雌性有四个卵巢发育阶段：一期（耗尽期）、二期（增殖期）、三期（早熟期）和四期（成熟期）。在每个发育阶段，卵巢在宏观上大小和颜色各异，在微观上，卵巢各阶段在卵原细胞和次级卵母细胞的比例上有所不同。在卵巢一期，卵巢主要包含卵原细胞、初级卵母细胞和Oc1。在二期，主要包含Oc1、Oc2和Oc3，而在三期，主要细胞是Oc4。成熟卵母细胞在四期同步出现。
