State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing, China.
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing, China.
Biosens Bioelectron. 2015 Jun 15;68:529-535. doi: 10.1016/j.bios.2015.01.052. Epub 2015 Jan 22.
This paper reports a novel dielectrophoresis (DEP) based method for manipulating individual living cells by modulating phase difference of electrical signals applied on DEP electrodes. A novel microchip with an array structure is also proposed, consisting of a plurality of quadrupole-electrode units patterned into array on a glass substrate with a pair of center electrodes locating at the center of each quadrupole-electrode unit. Living cells can be trapped and positioned at the center of each quadrupole-electrode unit by using negative DEP (nDEP) manipulation and form an array. The trapped cells in the array can be controllably moved from one position to another and even from one of quadrupole-electrode units to adjacent unit by changing the phase difference of the signals applied on the two pairs of opposite electrodes in each quadrupole-electrode unit. The microchip allows an efficient and flexible manipulation of individual living cells that can be applied to study single cells. The experiments are performed to verify that different types of cells (MCF-7 cell and HeLa cell) can be effectively distinguished between each other using the method without label and fluorometric measurements. An identification of individual living cell from dead cells is also well demonstrated.
本文报道了一种通过调制施加在介电泳（DEP）电极上的电信号的相位差来操纵单个活细胞的新型DEP 方法。还提出了一种具有阵列结构的新型微芯片，该微芯片由多个四极电极单元组成，这些单元以阵列形式图案化在玻璃基板上，一对中心电极位于每个四极电极单元的中心。通过使用负介电泳（nDEP）操纵将活细胞捕获并定位在每个四极电极单元的中心，并形成一个阵列。通过改变施加在每个四极电极单元的两对相对电极上的信号的相位差，可以将阵列中的捕获细胞从一个位置可控地移动到另一个位置，甚至从一个四极电极单元移动到相邻单元。该微芯片允许对单个活细胞进行高效灵活的操纵，可用于研究单细胞。实验验证了该方法无需标记和荧光测量即可有效区分不同类型的细胞（MCF-7 细胞和 HeLa 细胞）。还很好地证明了从死细胞中识别单个活细胞。
