Wang Yanjuan, Tong Ning, Li Fengqi, Zhao Kai, Wang Deguang, Niu Yijie, Xu Fengqiang, Cheng Jiale, Wang Junsheng
Software Institute, Dalian Jiaotong University, Dalian 116028, China.
Liaoning Key Laboratory of Marine Sensing and Intelligent Detection, College of Information Science and Technology, Dalian Maritime University, Dalian 116026, China.
Micromachines (Basel). 2023 Jan 8;14(1):159. doi: 10.3390/mi14010159.
Precise trap and manipulation of individual cells is a prerequisite for single-cell analysis, which has a wide range of applications in biology, chemistry, medicine, and materials. Herein, a microfluidic trapping system with a 3D electrode based on AC dielectrophoresis (DEP) technology is proposed, which can achieve the precise trapping and release of specific microparticles. The 3D electrode consists of four rectangular stereoscopic electrodes with an acute angle near the trapping chamber. It is made of Ag-PDMS material, and is the same height as the channel, which ensures the uniform DEP force will be received in the whole channel space, ensuring a better trapping effect can be achieved. The numerical simulation was conducted in terms of electrode height, angle, and channel width. Based on the simulation results, an optimal chip structure was obtained. Then, the polystyrene particles with different diameters were used as the samples to verify the effectiveness of the designed trapping system. The findings of this research will contribute to the application of cell trapping and manipulation, as well as single-cell analysis.
精确捕获和操控单个细胞是单细胞分析的前提条件，单细胞分析在生物学、化学、医学和材料领域有着广泛应用。在此，提出了一种基于交流介电泳（DEP）技术的带有三维电极的微流控捕获系统，该系统能够实现特定微颗粒的精确捕获和释放。三维电极由四个在捕获腔室附近带有锐角的矩形立体电极组成。它由银 - 聚二甲基硅氧烷（Ag - PDMS）材料制成，与通道高度相同，这确保了在整个通道空间内能够接收到均匀的DEP力，从而确保可以实现更好的捕获效果。针对电极高度、角度和通道宽度进行了数值模拟。基于模拟结果，获得了最优的芯片结构。然后，使用不同直径的聚苯乙烯颗粒作为样本，以验证所设计捕获系统的有效性。本研究结果将有助于细胞捕获与操控以及单细胞分析的应用。
