Pan Jian-Gang, Jiao Hai-Hua, Bai Zhi-Hui, Qi Hong-Yan, Ma An-Zhou, Zhuang Guo-qiang, Zhang Hong-xun
Huan Jing Ke Xue. 2014 Nov;35(11):4329-37.
The dynamics of rhizospheric fungal diversity and biomass at different sampling stages associated with two transgenic insectresistant cottons expressing Cry1Ac protein and their control varieties were studied under greenhouse conditions, followed by PCR-denaturing gradient gel electrophoresis (PCR-DGGE) and quantitative real-time polymerase chain reaction (Q-PCR), in order to evaluate the ecological security of planting transgenic cotton expressing Cry1Ac protein. The results indicated that the fungal superior bands in rhizosphere of transgenic Bt cotton were similar with that of control cotton at four sampling stages, the more obvious difference in the blurred bands among transgenic Bt cotton, JM20 and SHIYUAN321 was detected. The rhizospheric fungal biomass of transgenic Bt cotton SGK321 was significantly lower than that of its parental control cotton at seedling stage, while the slight decrease in fungal biomass of transgenic Bt cotton XP188 was detected at boll forming stage, the ill-defined decrease, even growing tendency in two transgenic Bt cottons was detected at other stages. However, the difference of rhizospheric fungal community compositions and biomass was not only existed between transgenic cotton and its control, but also between SHIYUAN321 and JM20, and the same phenomenon was also detected between transgenic Bt cotton SGK321 and XP188. Hence, Bt protein is not the only incentive resulting in the difference in fungal community composition and diversity, the decrease in biomass between transgenic cotton and untransgenic cotton, different cotton varieties has an effect on them.
在温室条件下，研究了两个表达Cry1Ac蛋白的转基因抗虫棉及其对照品种在不同采样阶段根际真菌多样性和生物量的动态变化，随后采用聚合酶链式反应-变性梯度凝胶电泳（PCR-DGGE）和实时荧光定量聚合酶链反应（Q-PCR），以评估种植表达Cry1Ac蛋白的转基因棉花的生态安全性。结果表明，在四个采样阶段，转基因Bt棉根际真菌优势条带与对照棉相似，但检测到转基因Bt棉、JM20和石远321之间模糊条带的差异更明显。转基因Bt棉SGK321在苗期根际真菌生物量显著低于其亲本对照棉，而转基因Bt棉XP188在结铃期真菌生物量略有下降，在其他阶段，两种转基因Bt棉真菌生物量下降不明显甚至有增长趋势。然而，根际真菌群落组成和生物量的差异不仅存在于转基因棉及其对照之间，也存在于石远321和JM20之间，转基因Bt棉SGK321和XP188之间也检测到同样的现象。因此，Bt蛋白不是导致真菌群落组成和多样性差异的唯一诱因，转基因棉与非转基因棉、不同棉花品种之间生物量的下降对其有影响。
