评估一种用于强化食品（面粉、糖和牛奶）中维生素A定量分析以进行质量控制的便携式设备。

Assessment of a portable device to quantify vitamin A in fortified foods (flour, sugar, and milk) for quality control.

作者信息

Laillou Arnaud, Renaud Cécile, Berger Jacques, Moench-Pfanner Regina, Fontan Laura, Avallone Sylvie

出版信息

Food Nutr Bull. 2014 Dec;35(4):449-57. doi: 10.1177/156482651403500407.

DOI:10.1177/156482651403500407

PMID:25639130

Abstract

BACKGROUND

Simple-to-use quantitative methods are needed to check the adequacy of vitamin A fortification levels.

OBJECTIVE

To assess the capacity of a portable fluorometer (iCheck FLUORO) and its test kit vials (iEx Mila) to quantify retinyl palmitate in fortified milks, flours (wheat, maize), and sugar.

METHODS

The portable fluorometer was assessed in a three-step procedure to determine its working range and linearity, intra-assay precision, and interperson precision. Measurements were compared with the results obtained by high-performance liquid chromatography (HPLC), commonly regarded as the standard method for vitamin A analysis.

RESULTS

The portable fluorometer (iCheck FLUORO) and its test kit vials (iEx Mila) precisely determined the vitamin A contents in fortifed flours, sugar, and milks. Its working range was 1 to 10, 0.5 to 3.0, and 5 to 15 mg retinol equivalents (RE) kg(-1) for flours (wheat and corn), milks, and sugar, respectively; these values are in accordance with the World Health Organization recommendations for food fortification in least developed countries. The limits of detection are higher than those of HPLC but are all satisfactory (< 1.46 mg RE kg(-1)). The coefficients of variation within and between observers were satisfactory, especially for sugar and milk.

CONCLUSIONS

The linear relationship between the data from the portable fluorometer and the HPLC data confirms that the portable fluorometer provides a good determination of the vitamin A content of the fortified products in the tested range.

摘要

背景

需要简单易用的定量方法来检查维生素A强化水平是否充足。

目的

评估便携式荧光计（iCheck FLUORO）及其试剂盒小瓶（iEx Mila）对强化牛奶、面粉（小麦、玉米）和糖中棕榈酸视黄酯进行定量的能力。

方法

通过三步程序对便携式荧光计进行评估，以确定其工作范围和线性、批内精密度和人际精密度。将测量结果与高效液相色谱法（HPLC）获得的结果进行比较，HPLC通常被视为维生素A分析的标准方法。

结果

便携式荧光计（iCheck FLUORO）及其试剂盒小瓶（iEx Mila）精确测定了强化面粉、糖和牛奶中的维生素A含量。其工作范围分别为面粉（小麦和玉米）1至10 mg视黄醇当量（RE）/kg、牛奶0.5至3.0 mg RE/kg、糖5至15 mg RE/kg；这些数值符合世界卫生组织对最不发达国家食品强化的建议。检测限高于HPLC，但均令人满意（<1.46 mg RE/kg）。观察者内部和观察者之间的变异系数令人满意，尤其是对于糖和牛奶。