Baldissera Matheus D, Pivoto Felipe L, Bottari Nathieli B, Tonin Alexandre A, Machado Gustavo, Aires Adelina R, Rocha José F X, Pelinson Luana P, Dalenogare Diéssica P, Schetinger Maria Rosa C, Morsch Vera M, Leal Marta L R, Da Silva Aleksandro S
Farmacology of Graduate Program, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Laboratory of Animal Endocrinology and Metabolism, Department of Large Animal Clinical, Hospital Veterinário Universitário, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
Exp Parasitol. 2015 Apr-May;151-152:34-8. doi: 10.1016/j.exppara.2015.01.010. Epub 2015 Jan 29.
Haemonchus contortus (order Strongylida) is a common parasitic nematode infecting small ruminants and causing significant economic losses worldwide. It induces innate and adaptive immune responses, which are essential for the clearance of this nematode from the host. Ecto-adenosine deaminase (E-ADA) is an enzyme that plays an important role in the immune system, while Zinc (Zn) has been found playing a critical role in E-ADA catalysis. Therefore, the aim of this study was to assess the effect of Zn supplementation on E-ADA activity in serum of lambs experimentally infected with H.contortus. To reach this purpose 28 male lambs (in average 25 kg) were used. The animals were divided into four groups: A and B composed of healthy animals (uninfected); C and D, infected with H.contortus. Groups B and D were supplemented with Zn Edetate, subcutaneously with 3 mg kg of live weight, on days 11 and 25 post-infection (PI). Blood and fecal samples were collected on the days 11, 25 and 39 PI, in order to assess hematocrit, seric E-ADA, and eggs per gram (EPG) counting, respectively. The animals of groups C and D showed severe hematocrit reduction (days 25 and 39 PI) and were EPG positive (days 11, 25 and 39 PI). On day 41 PI, three animals each group were subjected to necropsy. This procedure showed that animals of groups A and B did not have helminths in abomasum and intestines, while H.contortus were observed in groups C (5782.5 ± 810.9) and D (6185.0 ± 150.0). Infected and untreated animals (group C) showed a reduction in E-ADA activity, but this was not observed when the animals were supplemented with Zn (Group D). Therefore, based on our results, it was possible to observe that Zn supplementation exercised a positive effect on E-ADA activity in lambs infected with H.contortus, and did not allow a reduction in E-ADA activity, as occurred in the group infected and without supplementation. However, Zn supplementation was not able to prevent the worm burden.
捻转血矛线虫（圆线目）是一种常见的寄生线虫，感染小型反刍动物并在全球范围内造成重大经济损失。它会引发先天性和适应性免疫反应，这对于从宿主体内清除这种线虫至关重要。胞外腺苷脱氨酶（E-ADA）是一种在免疫系统中起重要作用的酶，而锌（Zn）已被发现对E-ADA催化起着关键作用。因此，本研究的目的是评估补充锌对实验感染捻转血矛线虫的羔羊血清中E-ADA活性的影响。为实现这一目的，使用了28只雄性羔羊（平均体重25千克）。动物被分为四组：A组和B组由健康动物（未感染）组成；C组和D组感染捻转血矛线虫。B组和D组在感染后第11天和第25天皮下注射乙二胺四乙酸锌，剂量为每千克体重3毫克。在感染后第11天、第25天和第39天采集血液和粪便样本，分别用于评估血细胞比容、血清E-ADA和每克粪便虫卵数（EPG）计数。C组和D组的动物在感染后第25天和第39天出现严重的血细胞比容降低，并且在感染后第11天、第25天和第39天EPG呈阳性。在感染后第41天，每组处死三只动物。该操作表明，A组和B组的动物在皱胃和肠道中没有蠕虫，而在C组（5782.5±810.9）和D组（6185.0±150.0）中观察到了捻转血矛线虫。未感染且未治疗的动物（C组）显示E-ADA活性降低，但在动物补充锌时（D组）未观察到这种情况。因此，根据我们的结果，可以观察到补充锌对感染捻转血矛线虫的羔羊的E-ADA活性有积极影响，并且不会像未补充锌的感染组那样导致E-ADA活性降低。然而，补充锌并不能防止虫体负担。
