Schlesinger M, Kincade P W
Hubert H. Humphrey Center for Experimental Medicine and Cancer Research, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Cell Immunol. 1989 Mar;119(1):22-9. doi: 10.1016/0008-8749(89)90220-7.
Thy-1 molecules have been shown to become anchored to the membrane of thymocytes and of T-cells via a phosphatidylinositol link. In the present study the intensity of immunofluorescent staining of mouse thymus cells by monoclonal xenoantibodies and alloantibodies specific for the Thy-1.2 determinant was significantly reduced following exposure of the cells to phosphatidylinositol-specific phospholipase C (PI-PLC). The majority of the PI-PLC-treated thymus cells retained, however, some reactivity with Thy-1.2 antibodies. In contrast, the immunofluorescent staining of thymus cells with monoclonal autoantibodies to Thy-1 determinants (20-10-5 and C16-31) was completely abolished by PI-PLC treatment. These results suggest that whereas monoclonal autoantibodies to Thy-1 react preferentially with PI-PLC-sensitive Thy-1 molecules, monoclonal antibodies to the Thy-1.2 specificity react with all cell surface Thy-1 molecules, regardless of their sensitivity to PI-PLC treatment.
已证明Thy-1分子通过磷脂酰肌醇连接锚定在胸腺细胞和T细胞的膜上。在本研究中，小鼠胸腺细胞经针对Thy-1.2决定簇的单克隆异种抗体和同种抗体进行免疫荧光染色后，将细胞暴露于磷脂酰肌醇特异性磷脂酶C（PI-PLC）后，染色强度显著降低。然而，大多数经PI-PLC处理的胸腺细胞仍与Thy-1.2抗体有一定反应性。相比之下，用针对Thy-1决定簇的单克隆自身抗体（20-10-5和C16-31）对胸腺细胞进行免疫荧光染色，经PI-PLC处理后完全消失。这些结果表明，针对Thy-1的单克隆自身抗体优先与对PI-PLC敏感的Thy-1分子反应，而针对Thy-1.2特异性的单克隆抗体则与所有细胞表面的Thy-1分子反应，无论它们对PI-PLC处理的敏感性如何。
