College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
College of Chemistry, Jilin University, 2699 Qianjin Street, Changchun 130012, PR China.
Talanta. 2015 Apr;135:163-9. doi: 10.1016/j.talanta.2014.12.049. Epub 2015 Jan 8.
A novel microwave-assisted homogeneous ionic liquid microextraction (MA-HILME) method was first developed for the extraction of Sudan dyes in red wines followed by detection using high-performance liquid chromatography. 1-dodecyl-3-methylimidazolium bromide ([C12MIM]Br) was used as extractant in a microwave-assisted extraction (MAE) procedure, and then transferred into hydrophobic solid-state ionic liquid after adding ammonium hexafluorophosphate ([NH4][PF6]). The separation between liquid state sample and solid state extractant can be realized easily. Several experimental parameters, including type and amount of extraction solvent, microwave power and irradiation time, amount of ion-exchange reagent ([NH4][PF6]), pH of sample solution, and ionic strength, were evaluated. Under the optimum experimental conditions, the linearity for the determining of the analytes was in the range of 0.5-100 μg L(-1), with the correlation coefficients ranging from 0.9995 to 0.9999. The limits of detection for Sudan I, Sudan II, Sudan III, and Sudan IV were 0.19, 0.18, 0.24, and 0.16 μg L(-1), respectively. When the present method was applied to the determination of Sudan dyes in red wines, satisfactory recoveries were obtained in the range of 78.5-106.8%, and relative standard deviations were lower than 9.7%.
首次建立了一种新型的微波辅助均相离子液体微萃取（MA-HILME）方法，用于萃取红酒中的苏丹染料，然后使用高效液相色谱法进行检测。在微波辅助萃取（MAE）过程中，使用 1-十二烷基-3-甲基咪唑溴盐（[C12MIM]Br）作为萃取剂，然后在加入六氟磷酸铵（[NH4][PF6]）后转化为疏水性固态离子液体。可以轻松实现液相样品和固相萃取剂之间的分离。评估了几种实验参数，包括萃取溶剂的类型和用量、微波功率和辐射时间、离子交换试剂（[NH4][PF6]）的用量、样品溶液的 pH 值和离子强度。在最佳实验条件下，分析物的测定线性范围为 0.5-100μg L（-1），相关系数范围为 0.9995 至 0.9999。苏丹红 I、苏丹红 II、苏丹红 III 和苏丹红 IV 的检出限分别为 0.19、0.18、0.24 和 0.16μg L（-1）。当将本方法应用于红酒中苏丹染料的测定时，回收率在 78.5-106.8%范围内，相对标准偏差低于 9.7%。
