电压门控离子通道的逐个原子工程：对功能和药理学的深入洞察
Atom-by-atom engineering of voltage-gated ion channels: magnified insights into function and pharmacology.
作者信息
Pless Stephan A, Kim Robin Y, Ahern Christopher A, Kurata Harley T
机构信息
Center for Biopharmaceuticals, Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
Department of Anesthesiology, Pharmacology, and Therapeutics, University of British Columbia, Vancouver, BC, Canada.
出版信息
J Physiol. 2015 Jun 15;593(12):2627-34. doi: 10.1113/jphysiol.2014.287714. Epub 2015 Mar 13.
Abstract
Unnatural amino acid incorporation into ion channels has proven to be a valuable approach to interrogate detailed hypotheses arising from atomic resolution structures. In this short review, we provide a brief overview of some of the basic principles and methods for incorporation of unnatural amino acids into proteins. We also review insights into the function and pharmacology of voltage-gated ion channels that have emerged from unnatural amino acid mutagenesis approaches.
摘要
将非天然氨基酸掺入离子通道已被证明是一种用于探究源自原子分辨率结构的详细假设的宝贵方法。在这篇简短的综述中，我们简要概述了将非天然氨基酸掺入蛋白质的一些基本原理和方法。我们还回顾了从非天然氨基酸诱变方法中获得的关于电压门控离子通道功能和药理学的见解。
相似文献
1
Atom-by-atom engineering of voltage-gated ion channels: magnified insights into function and pharmacology.电压门控离子通道的逐个原子工程：对功能和药理学的深入洞察
J Physiol. 2015 Jun 15;593(12):2627-34. doi: 10.1113/jphysiol.2014.287714. Epub 2015 Mar 13.
2
Site-Directed Unnatural Amino Acid Mutagenesis to Investigate Potassium Channel Pharmacology in Xenopus laevis Oocytes.利用定点非天然氨基酸诱变技术研究非洲爪蟾卵母细胞中的钾通道药理学
Methods Mol Biol. 2018;1684:253-263. doi: 10.1007/978-1-4939-7362-0_19.
3
Unnatural amino acid mutagenesis in mapping ion channel function.用于绘制离子通道功能图谱的非天然氨基酸诱变
Curr Opin Neurobiol. 2003 Jun;13(3):264-70. doi: 10.1016/s0959-4388(03)00068-0.
4
Probing Ion Channel Structure and Function Using Light-Sensitive Amino Acids.用光敏感氨基酸探测离子通道结构和功能。
Trends Biochem Sci. 2018 Jun;43(6):436-451. doi: 10.1016/j.tibs.2018.02.012. Epub 2018 Apr 10.
5
Single amino acids in the carboxyl terminal domain of aquaporin-1 contribute to cGMP-dependent ion channel activation.水通道蛋白-1羧基末端结构域中的单个氨基酸有助于环磷酸鸟苷依赖性离子通道的激活。
BMC Physiol. 2003 Oct 15;3:12. doi: 10.1186/1472-6793-3-12.
6
Origin of functional diversity among tetrameric voltage-gated channels.四聚体电压门控通道功能多样性的起源。
Proteins. 2007 Jan 1;66(1):136-46. doi: 10.1002/prot.21187.
7
International Union of Pharmacology. XLII. Compendium of voltage-gated ion channels: cyclic nucleotide-modulated channels.国际药理学联合会。XLII. 电压门控离子通道纲要：环核苷酸调节通道。
Pharmacol Rev. 2003 Dec;55(4):587-9. doi: 10.1124/pr.55.4.10.
8
The S4-S5 linker couples voltage sensing and activation of pacemaker channels.S4-S5连接体将电压传感与起搏通道的激活相耦合。
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11277-82. doi: 10.1073/pnas.201250598. Epub 2001 Sep 11.
9
10
Three amino acids in the C-linker are major determinants of gating in cyclic nucleotide-gated channels.C连接子中的三个氨基酸是环核苷酸门控通道门控的主要决定因素。
EMBO J. 1998 Jan 15;17(2):353-62. doi: 10.1093/emboj/17.2.353.
引用本文的文献
1
Improved ANAP incorporation and VCF analysis reveal details of P2X7 current facilitation and a limited conformational interplay between ATP binding and the intracellular ballast domain.改进的 ANAP 掺入和 VCF 分析揭示了 P2X7 电流易化的细节以及 ATP 结合和细胞内压载域之间有限的构象相互作用。
Elife. 2023 Jan 4;12:e82479. doi: 10.7554/eLife.82479.
2
Exploring structural dynamics of a membrane protein by combining bioorthogonal chemistry and cysteine mutagenesis.通过结合生物正交化学和半胱氨酸突变技术探索膜蛋白的结构动力学。
Elife. 2019 Nov 12;8:e50776. doi: 10.7554/eLife.50776.
3
NIPS-JP symposium: cutting-edge approaches towards the functioning mechanisms of membrane proteins.NIPS-JP研讨会：针对膜蛋白功能机制的前沿方法
J Physiol. 2015 Jun 15;593(12):2551-2. doi: 10.1113/JP270762.
本文引用的文献
1
Efficient multisite unnatural amino acid incorporation in mammalian cells via optimized pyrrolysyl tRNA synthetase/tRNA expression and engineered eRF1.通过优化吡咯赖氨酸tRNA合成酶/tRNA表达和工程化eRF1，在哺乳动物细胞中高效进行多位点非天然氨基酸掺入。
J Am Chem Soc. 2014 Nov 5;136(44):15577-83. doi: 10.1021/ja5069728. Epub 2014 Oct 28.
2
Genetic code expansion and bioorthogonal labelling enables cell specific proteomics in an animal.遗传密码扩展和生物正交标记使动物中的细胞特异性蛋白质组学成为可能。
Curr Opin Chem Biol. 2014 Aug;21:154-60. doi: 10.1016/j.cbpa.2014.07.001. Epub 2014 Aug 20.
3
In vivo incorporation of non-canonical amino acids by using the chemical aminoacylation strategy: a broadly applicable mechanistic tool.利用化学氨酰化策略在体内掺入非标准氨基酸：一种广泛适用的机制工具。
Chembiochem. 2014 Aug 18;15(12):1710-20. doi: 10.1002/cbic.201402080. Epub 2014 Jul 2.
4
Asymmetric functional contributions of acidic and aromatic side chains in sodium channel voltage-sensor domains.酸性和芳香族侧链在钠离子通道电压传感器域中的非对称功能贡献。
J Gen Physiol. 2014 May;143(5):645-56. doi: 10.1085/jgp.201311036.
5
Genetically encoding a light switch in an ionotropic glutamate receptor reveals subunit-specific interfaces.在离子型谷氨酸受体中遗传编码光开关揭示了亚基特异性界面。
Proc Natl Acad Sci U S A. 2014 Apr 22;111(16):6081-6. doi: 10.1073/pnas.1318808111. Epub 2014 Apr 8.
6
Photoinactivation of glutamate receptors by genetically encoded unnatural amino acids.通过基因编码的非天然氨基酸对谷氨酸受体进行光灭活。
J Neurosci. 2014 Jan 15;34(3):980-91. doi: 10.1523/JNEUROSCI.3725-13.2014.
7
Hydrogen bonds as molecular timers for slow inactivation in voltage-gated potassium channels.氢键作为电压门控钾通道缓慢失活的分子定时器。
Elife. 2013 Dec 10;2:e01289. doi: 10.7554/eLife.01289.
8
In vivo expression of a light-activatable potassium channel using unnatural amino acids.利用非天然氨基酸在体内表达光激活钾通道。
Neuron. 2013 Oct 16;80(2):358-70. doi: 10.1016/j.neuron.2013.08.016.
9
Using protein backbone mutagenesis to dissect the link between ion occupancy and C-type inactivation in K+ channels.利用蛋白主链突变技术解析 K+ 通道中离子占据与 C 型失活之间的联系。
Proc Natl Acad Sci U S A. 2013 Oct 29;110(44):17886-91. doi: 10.1073/pnas.1314356110. Epub 2013 Oct 15.
10
Semisynthetic K+ channels show that the constricted conformation of the selectivity filter is not the C-type inactivated state.半合成钾通道表明，选择性过滤器的紧缩构象不是 C 型失活状态。
Proc Natl Acad Sci U S A. 2013 Sep 24;110(39):15698-703. doi: 10.1073/pnas.1308699110. Epub 2013 Sep 9.
Zotero 插件